Effects of Spirulina on Cyclophosphamide-Induced Ovarian Toxicity in Rats: Biochemical and Histomorphometric Evaluation of the Ovary
Cyclophosphamide (Cyc) is known to cause ovotoxicity and infertility in women. Our aim is to investigate the possible ovotoxic effects of Cyc and possible antioxidant and protective effects of blue-green algae, Spirulina (Sp), in rat ovaries. Eighteen rats were given: group I (n=6, control); group I...
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Online Access: | http://dx.doi.org/10.1155/2013/764262 |
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doaj-f07673bdac164f51985e6da984f8833d2020-11-24T23:53:50ZengHindawi LimitedBiochemistry Research International2090-22472090-22552013-01-01201310.1155/2013/764262764262Effects of Spirulina on Cyclophosphamide-Induced Ovarian Toxicity in Rats: Biochemical and Histomorphometric Evaluation of the OvaryNese Arzu Yener0Orhun Sinanoglu1Erdin Ilter2Aygen Celik3Gulbuz Sezgin4Ahmet Midi5Ugur Deveci6Fehime Aksungar7Maltepe University School of Medicine, Department of Pathology, Maltepe, 34843 Istanbul, TurkeyMaltepe University School of Medicine, Department of Urology, Maltepe, 34843 Istanbul, TurkeyMaltepe University School of Medicine, Department of Obstetrics & Gynecology, Maltepe, 34843 Istanbul, TurkeyMaltepe University School of Medicine, Department of Obstetrics & Gynecology, Maltepe, 34843 Istanbul, TurkeyMaltepe University School of Medicine, Department of Internal Medicine, Maltepe, 34843 Istanbul, TurkeyMaltepe University School of Medicine, Department of Pathology, Maltepe, 34843 Istanbul, TurkeyMaltepe University School of Medicine, Department of General Surgery, Maltepe, 34843 Istanbul, TurkeyMaltepe University School of Medicine, Department of Biochemistry, Maltepe, 34843 Istanbul, TurkeyCyclophosphamide (Cyc) is known to cause ovotoxicity and infertility in women. Our aim is to investigate the possible ovotoxic effects of Cyc and possible antioxidant and protective effects of blue-green algae, Spirulina (Sp), in rat ovaries. Eighteen rats were given: group I (n=6, control); group II (n=6, CP), a single dose Cyc; group III (n=6, Sp+Cyc), 7 days Sp+single dose Cyc. Tissue malondialdehyde (MDA) levels, superoxide dismutase (SOD), and catalase (CAT) activities are assessed biochemically. Normal and atretic primordial and primary follicle counts for all sections obtained for each ovary are calculated. Mean number of follicle counts for each group are compared. In Sp+Cyc group, tissue MDA levels were significantly lower than those in the CP and higher than those in the C group (CP>Sp+Cyc>C). Tissue SOD activity was significantly higher in Sp+Cyc group than that in the CP group and lower than that in the C group (C>Sp+Cyc>C). No statistically significant difference was found between the ovarian CAT activities in any group. Histomorphometrically, there was also no significant difference between the mean numbers of normal and atretic small follicle counts. Our results suggest that single dose Cyc has adverse effects on oxidant status of the ovaries and Sp has protective effects in Cyc-induced ovotoxicity.http://dx.doi.org/10.1155/2013/764262 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nese Arzu Yener Orhun Sinanoglu Erdin Ilter Aygen Celik Gulbuz Sezgin Ahmet Midi Ugur Deveci Fehime Aksungar |
spellingShingle |
Nese Arzu Yener Orhun Sinanoglu Erdin Ilter Aygen Celik Gulbuz Sezgin Ahmet Midi Ugur Deveci Fehime Aksungar Effects of Spirulina on Cyclophosphamide-Induced Ovarian Toxicity in Rats: Biochemical and Histomorphometric Evaluation of the Ovary Biochemistry Research International |
author_facet |
Nese Arzu Yener Orhun Sinanoglu Erdin Ilter Aygen Celik Gulbuz Sezgin Ahmet Midi Ugur Deveci Fehime Aksungar |
author_sort |
Nese Arzu Yener |
title |
Effects of Spirulina on Cyclophosphamide-Induced Ovarian Toxicity in Rats: Biochemical and Histomorphometric Evaluation of the Ovary |
title_short |
Effects of Spirulina on Cyclophosphamide-Induced Ovarian Toxicity in Rats: Biochemical and Histomorphometric Evaluation of the Ovary |
title_full |
Effects of Spirulina on Cyclophosphamide-Induced Ovarian Toxicity in Rats: Biochemical and Histomorphometric Evaluation of the Ovary |
title_fullStr |
Effects of Spirulina on Cyclophosphamide-Induced Ovarian Toxicity in Rats: Biochemical and Histomorphometric Evaluation of the Ovary |
title_full_unstemmed |
Effects of Spirulina on Cyclophosphamide-Induced Ovarian Toxicity in Rats: Biochemical and Histomorphometric Evaluation of the Ovary |
title_sort |
effects of spirulina on cyclophosphamide-induced ovarian toxicity in rats: biochemical and histomorphometric evaluation of the ovary |
publisher |
Hindawi Limited |
series |
Biochemistry Research International |
issn |
2090-2247 2090-2255 |
publishDate |
2013-01-01 |
description |
Cyclophosphamide (Cyc) is known to cause ovotoxicity and infertility in women. Our aim is to investigate the possible ovotoxic effects of Cyc and possible antioxidant and protective effects of blue-green algae, Spirulina (Sp), in rat ovaries. Eighteen rats were given: group I (n=6, control); group II (n=6, CP), a single dose Cyc; group III (n=6, Sp+Cyc), 7 days Sp+single dose Cyc. Tissue malondialdehyde (MDA) levels, superoxide dismutase (SOD), and catalase (CAT) activities are assessed biochemically. Normal and atretic primordial and primary follicle counts for all sections obtained for each ovary are calculated. Mean number of follicle counts for each group are compared. In Sp+Cyc group, tissue MDA levels were significantly lower than those in the CP and higher than those in the C group (CP>Sp+Cyc>C). Tissue SOD activity was significantly higher in Sp+Cyc group than that in the CP group and lower than that in the C group (C>Sp+Cyc>C). No statistically significant difference was found between the ovarian CAT activities in any group. Histomorphometrically, there was also no significant difference between the mean numbers of normal and atretic small follicle counts. Our results suggest that single dose Cyc has adverse effects on oxidant status of the ovaries and Sp has protective effects in Cyc-induced ovotoxicity. |
url |
http://dx.doi.org/10.1155/2013/764262 |
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