Proven and less studied hematopoietic and vasoactive growth factors in retinal capillary hemangioma

Pathogenesis of retinal capillary hemangioma has not been sufficiently studied at the present time. Therefore, the study of cytokine levels in biological fluids seems to be very relevant in order to increase knowledge about the mechanisms of the disease development and searching for targeted therapi...

Full description

Bibliographic Details
Main Authors: V. V. Neroev, N. V. Balatskaya, A. Yu. Novikova, M. V. Ryabina, P. A. Ilyukhin
Format: Article
Language:Russian
Published: SPb RAACI 2020-12-01
Series:Medicinskaâ Immunologiâ
Subjects:
Online Access:https://www.mimmun.ru/mimmun/article/view/2002
id doaj-f123eff217614662834c83b721d64ed4
record_format Article
spelling doaj-f123eff217614662834c83b721d64ed42021-07-29T09:02:36ZrusSPb RAACIMedicinskaâ Immunologiâ1563-06252313-741X2020-12-0122594395610.15789/1563-0625-PAL-20021322Proven and less studied hematopoietic and vasoactive growth factors in retinal capillary hemangiomaV. V. Neroev0N. V. Balatskaya1A. Yu. Novikova2M. V. Ryabina3P. A. Ilyukhin4Helmholtz National Medical Research Center of Eye DiseasesHelmholtz National Medical Research Center of Eye DiseasesHelmholtz National Medical Research Center of Eye DiseasesHelmholtz National Medical Research Center of Eye DiseasesHelmholtz National Medical Research Center of Eye DiseasesPathogenesis of retinal capillary hemangioma has not been sufficiently studied at the present time. Therefore, the study of cytokine levels in biological fluids seems to be very relevant in order to increase knowledge about the mechanisms of the disease development and searching for targeted therapies. The content of hematopoietic and vasoactive growth factors in blood serum, lacrimal fluid, and vitreous body was studied in patients with retinal capillary hemangioma. A total of 26 patients with retinal angiomatosis were examined. The samples of blood serum (n = 23) and lacrimal fluid (n = 10) from practically healthy people aged 22 to 46 (27.4±1.4 years) were used as a control. To perform comparative assessment of cytokine concentrations in the vitreous body of patients with retinal capillary hemangioma, were used samples of the vitreous body from 6 patients (average age 33±4.7 years; from 21 to 49 years) with rhegmatogenous retinal detachment. To measure the cytokine concentrations, we applied multiplex analysis technique using the xMAP platform with LuminexxPONENT 3.1 program and ProcartaPlex sets (eBioscience, Austria). A detailed characteristic of vasoactive factors in capillary retinal hemangioma was obtained as a result of this work. Some disorders in chemokine regulation were identified. There was a significant increase in serum concentrations of three vasoactive factors, i.e., PDGF-BB, HGF, and PIGF-1, with a decrease in chemokines (MCP-1, MIP-1α, and MIP-1β). The frequencies of PIGF-1 and MIP-1α detection also significantly differed from the control group. SCF was significantly more often determined in patients with retinal angiomatosis only at the systemic level. Correlations between PDGF-BB and PIGF-1, as well as PIGF-1 and MIP-1β were shown. A significant increase in VEGF-A, HGF, VEGF-D, as well as MCP-1 concentrations was shown in the lacrimal fluid. The inversion of PDGF-BB concentrations in serum and lacrimal fluid was noted. Analysis of intraocular cytokine levels revealed a significant increase in VEGF-A and HGF concentrations, with marked decrease in MIP-1α and MIP-1β. PDGF-BB in 100% of cases was determined only in vitreous body of patients with retinal angiomatosis. With respect to the revealed characteristic shifts of HGF/SF intraocular production in retinal capillary hemangioma, it seems relevant to search ways for its inhibition, thus providing potential basis for a new therapeutic strategy in treatment of retinal angiomatosis.https://www.mimmun.ru/mimmun/article/view/2002retinal capillary hemangiomavhl genevon hippel-lindau syndromepathogenesiscytokines
collection DOAJ
language Russian
format Article
sources DOAJ
author V. V. Neroev
N. V. Balatskaya
A. Yu. Novikova
M. V. Ryabina
P. A. Ilyukhin
spellingShingle V. V. Neroev
N. V. Balatskaya
A. Yu. Novikova
M. V. Ryabina
P. A. Ilyukhin
Proven and less studied hematopoietic and vasoactive growth factors in retinal capillary hemangioma
Medicinskaâ Immunologiâ
retinal capillary hemangioma
vhl gene
von hippel-lindau syndrome
pathogenesis
cytokines
author_facet V. V. Neroev
N. V. Balatskaya
A. Yu. Novikova
M. V. Ryabina
P. A. Ilyukhin
author_sort V. V. Neroev
title Proven and less studied hematopoietic and vasoactive growth factors in retinal capillary hemangioma
title_short Proven and less studied hematopoietic and vasoactive growth factors in retinal capillary hemangioma
title_full Proven and less studied hematopoietic and vasoactive growth factors in retinal capillary hemangioma
title_fullStr Proven and less studied hematopoietic and vasoactive growth factors in retinal capillary hemangioma
title_full_unstemmed Proven and less studied hematopoietic and vasoactive growth factors in retinal capillary hemangioma
title_sort proven and less studied hematopoietic and vasoactive growth factors in retinal capillary hemangioma
publisher SPb RAACI
series Medicinskaâ Immunologiâ
issn 1563-0625
2313-741X
publishDate 2020-12-01
description Pathogenesis of retinal capillary hemangioma has not been sufficiently studied at the present time. Therefore, the study of cytokine levels in biological fluids seems to be very relevant in order to increase knowledge about the mechanisms of the disease development and searching for targeted therapies. The content of hematopoietic and vasoactive growth factors in blood serum, lacrimal fluid, and vitreous body was studied in patients with retinal capillary hemangioma. A total of 26 patients with retinal angiomatosis were examined. The samples of blood serum (n = 23) and lacrimal fluid (n = 10) from practically healthy people aged 22 to 46 (27.4±1.4 years) were used as a control. To perform comparative assessment of cytokine concentrations in the vitreous body of patients with retinal capillary hemangioma, were used samples of the vitreous body from 6 patients (average age 33±4.7 years; from 21 to 49 years) with rhegmatogenous retinal detachment. To measure the cytokine concentrations, we applied multiplex analysis technique using the xMAP platform with LuminexxPONENT 3.1 program and ProcartaPlex sets (eBioscience, Austria). A detailed characteristic of vasoactive factors in capillary retinal hemangioma was obtained as a result of this work. Some disorders in chemokine regulation were identified. There was a significant increase in serum concentrations of three vasoactive factors, i.e., PDGF-BB, HGF, and PIGF-1, with a decrease in chemokines (MCP-1, MIP-1α, and MIP-1β). The frequencies of PIGF-1 and MIP-1α detection also significantly differed from the control group. SCF was significantly more often determined in patients with retinal angiomatosis only at the systemic level. Correlations between PDGF-BB and PIGF-1, as well as PIGF-1 and MIP-1β were shown. A significant increase in VEGF-A, HGF, VEGF-D, as well as MCP-1 concentrations was shown in the lacrimal fluid. The inversion of PDGF-BB concentrations in serum and lacrimal fluid was noted. Analysis of intraocular cytokine levels revealed a significant increase in VEGF-A and HGF concentrations, with marked decrease in MIP-1α and MIP-1β. PDGF-BB in 100% of cases was determined only in vitreous body of patients with retinal angiomatosis. With respect to the revealed characteristic shifts of HGF/SF intraocular production in retinal capillary hemangioma, it seems relevant to search ways for its inhibition, thus providing potential basis for a new therapeutic strategy in treatment of retinal angiomatosis.
topic retinal capillary hemangioma
vhl gene
von hippel-lindau syndrome
pathogenesis
cytokines
url https://www.mimmun.ru/mimmun/article/view/2002
work_keys_str_mv AT vvneroev provenandlessstudiedhematopoieticandvasoactivegrowthfactorsinretinalcapillaryhemangioma
AT nvbalatskaya provenandlessstudiedhematopoieticandvasoactivegrowthfactorsinretinalcapillaryhemangioma
AT ayunovikova provenandlessstudiedhematopoieticandvasoactivegrowthfactorsinretinalcapillaryhemangioma
AT mvryabina provenandlessstudiedhematopoieticandvasoactivegrowthfactorsinretinalcapillaryhemangioma
AT pailyukhin provenandlessstudiedhematopoieticandvasoactivegrowthfactorsinretinalcapillaryhemangioma
_version_ 1721249685928148992