Responses of the Pheromone-Binding Protein of the Silk Moth <i>Bombyx Mori</i> on a Graphene Biosensor Match Binding Constants in Solution
An electronic biosensor for odors was assembled by immobilizing the silk moth <i>Bombyx mori</i> pheromone binding protein (BmorPBP1) on a reduced graphene oxide surface of a field-effect transistor. At physiological pH, the sensor detects the <i>B. mori</i> pheromones, bomby...
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doaj-f178e358d44e450bbfc80b3bcf512b9a2021-01-13T00:03:27ZengMDPI AGSensors1424-82202021-01-012149949910.3390/s21020499Responses of the Pheromone-Binding Protein of the Silk Moth <i>Bombyx Mori</i> on a Graphene Biosensor Match Binding Constants in SolutionCaroline Bonazza0Jiao Zhu1Roger Hasler2Rosa Mastrogiacomo3Paolo Pelosi4Wolfgang Knoll5CEST Competence Centre for Electrochemical Surface Technology, 2700 Wiener Neustadt, AustriaAustrian Institute of Technology GmbH, Biosensor Technologies, 3430 Tulln, AustriaAustrian Institute of Technology GmbH, Biosensor Technologies, 3430 Tulln, AustriaDepartment of Agriculture, Food, and Environment, University of Pisa, 56124 Pisa, ItalyAustrian Institute of Technology GmbH, Biosensor Technologies, 3430 Tulln, AustriaCEST Competence Centre for Electrochemical Surface Technology, 2700 Wiener Neustadt, AustriaAn electronic biosensor for odors was assembled by immobilizing the silk moth <i>Bombyx mori</i> pheromone binding protein (BmorPBP1) on a reduced graphene oxide surface of a field-effect transistor. At physiological pH, the sensor detects the <i>B. mori</i> pheromones, bombykol and bombykal, with good affinity and specificity. Among the other odorants tested, only eugenol elicited a strong signal, while terpenoids and other odorants (linalool, geraniol, isoamyl acetate, and 2-isobutyl-3-methoxypyrazine) produced only very weak responses. Parallel binding assays were performed with the same protein and the same ligands, using the common fluorescence approach adopted for similar proteins. The results are in good agreement with the sensor’s responses: bombykol and bombykal, together with eugenol, proved to be strong ligands, while the other compounds showed only poor affinity. When tested at pH 4, the protein failed to bind bombykol both in solution and when immobilized on the sensor. This result further indicates that the BmorPBP1 retains its full activity when immobilized on a surface, including the conformational change observed in acidic conditions. The good agreement between fluorescence assays and sensor responses suggests that ligand-binding assays in solution can be used to screen mutants of a binding protein when selecting the best form to be immobilized on a biosensor.https://www.mdpi.com/1424-8220/21/2/499biosensorfield-effect transistorgrapheneodorant-binding proteinglobal analysisfluorescence displacement assay |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Caroline Bonazza Jiao Zhu Roger Hasler Rosa Mastrogiacomo Paolo Pelosi Wolfgang Knoll |
spellingShingle |
Caroline Bonazza Jiao Zhu Roger Hasler Rosa Mastrogiacomo Paolo Pelosi Wolfgang Knoll Responses of the Pheromone-Binding Protein of the Silk Moth <i>Bombyx Mori</i> on a Graphene Biosensor Match Binding Constants in Solution Sensors biosensor field-effect transistor graphene odorant-binding protein global analysis fluorescence displacement assay |
author_facet |
Caroline Bonazza Jiao Zhu Roger Hasler Rosa Mastrogiacomo Paolo Pelosi Wolfgang Knoll |
author_sort |
Caroline Bonazza |
title |
Responses of the Pheromone-Binding Protein of the Silk Moth <i>Bombyx Mori</i> on a Graphene Biosensor Match Binding Constants in Solution |
title_short |
Responses of the Pheromone-Binding Protein of the Silk Moth <i>Bombyx Mori</i> on a Graphene Biosensor Match Binding Constants in Solution |
title_full |
Responses of the Pheromone-Binding Protein of the Silk Moth <i>Bombyx Mori</i> on a Graphene Biosensor Match Binding Constants in Solution |
title_fullStr |
Responses of the Pheromone-Binding Protein of the Silk Moth <i>Bombyx Mori</i> on a Graphene Biosensor Match Binding Constants in Solution |
title_full_unstemmed |
Responses of the Pheromone-Binding Protein of the Silk Moth <i>Bombyx Mori</i> on a Graphene Biosensor Match Binding Constants in Solution |
title_sort |
responses of the pheromone-binding protein of the silk moth <i>bombyx mori</i> on a graphene biosensor match binding constants in solution |
publisher |
MDPI AG |
series |
Sensors |
issn |
1424-8220 |
publishDate |
2021-01-01 |
description |
An electronic biosensor for odors was assembled by immobilizing the silk moth <i>Bombyx mori</i> pheromone binding protein (BmorPBP1) on a reduced graphene oxide surface of a field-effect transistor. At physiological pH, the sensor detects the <i>B. mori</i> pheromones, bombykol and bombykal, with good affinity and specificity. Among the other odorants tested, only eugenol elicited a strong signal, while terpenoids and other odorants (linalool, geraniol, isoamyl acetate, and 2-isobutyl-3-methoxypyrazine) produced only very weak responses. Parallel binding assays were performed with the same protein and the same ligands, using the common fluorescence approach adopted for similar proteins. The results are in good agreement with the sensor’s responses: bombykol and bombykal, together with eugenol, proved to be strong ligands, while the other compounds showed only poor affinity. When tested at pH 4, the protein failed to bind bombykol both in solution and when immobilized on the sensor. This result further indicates that the BmorPBP1 retains its full activity when immobilized on a surface, including the conformational change observed in acidic conditions. The good agreement between fluorescence assays and sensor responses suggests that ligand-binding assays in solution can be used to screen mutants of a binding protein when selecting the best form to be immobilized on a biosensor. |
topic |
biosensor field-effect transistor graphene odorant-binding protein global analysis fluorescence displacement assay |
url |
https://www.mdpi.com/1424-8220/21/2/499 |
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