Screening of iron-enriched fungus from natural environment and evaluation of organically bound iron bioavailability in rats
Iron is an essential element for nearly all living organisms, and its deficiency is the most common form of malnutrition in the world. The organic forms of trace elements are considered more bioavailable than the inorganic forms. Although Saccharomyces cerevisiae can enrich metal elements and conver...
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Sociedade Brasileira de Ciência e Tecnologia de Alimentos
2015-03-01
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doaj-f3cbf04e0fe94c2d9ba5f28676b9ed842020-11-24T23:50:57ZengSociedade Brasileira de Ciência e Tecnologia de AlimentosFood Science and Technology1678-457X2015-03-01351586510.1590/1678-457X.6454S0101-20612015000100058Screening of iron-enriched fungus from natural environment and evaluation of organically bound iron bioavailability in ratsXin-guo ZhangYi-nan PengXiao-ru LiGuo-di MaXiao-qian ChenIron is an essential element for nearly all living organisms, and its deficiency is the most common form of malnutrition in the world. The organic forms of trace elements are considered more bioavailable than the inorganic forms. Although Saccharomyces cerevisiae can enrich metal elements and convert inorganic iron to organic species, its tolerability and transforming capacity are limited. The aim of this study was to screen higher biomass and other iron-enriched fungi strains besides Saccharomyces cerevisiae from the natural environment. A PDA medium containing 800 μg/mL iron was used for initial screening. Fifty strains that tolerated high iron concentration were isolated from the natural environment, and only one strain, No.BY1109, grew well at Fe (II) concentration of 10,000μg/ml. According to morphological characterization, 18S rDNA sequence analysis, and biophysical and biochemical characterization, the strain No.BY1109 was identified as Rhodotorula. The iron content of No.BY1109 (10 mg Fe/g dry cell) was determined using atomic absorption spectrometry. The results of distribution of iron in the cells showed that iron ion was mainly chelated in the cell walls and vacuoles. The bioavailability in rats confirmed that strain No.BY1109 had higher absorption efficiency than that of ferrous sulfate after single dose oral administration. The present study introduces new iron supplements, and it is a basis for finding new iron supplements from natural environment.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612015000100058&lng=en&tlng=eniron-rich fungusscreening and isolationidentificationcell-iron distributionbioavailability |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xin-guo Zhang Yi-nan Peng Xiao-ru Li Guo-di Ma Xiao-qian Chen |
spellingShingle |
Xin-guo Zhang Yi-nan Peng Xiao-ru Li Guo-di Ma Xiao-qian Chen Screening of iron-enriched fungus from natural environment and evaluation of organically bound iron bioavailability in rats Food Science and Technology iron-rich fungus screening and isolation identification cell-iron distribution bioavailability |
author_facet |
Xin-guo Zhang Yi-nan Peng Xiao-ru Li Guo-di Ma Xiao-qian Chen |
author_sort |
Xin-guo Zhang |
title |
Screening of iron-enriched fungus from natural environment and evaluation of organically bound iron bioavailability in rats |
title_short |
Screening of iron-enriched fungus from natural environment and evaluation of organically bound iron bioavailability in rats |
title_full |
Screening of iron-enriched fungus from natural environment and evaluation of organically bound iron bioavailability in rats |
title_fullStr |
Screening of iron-enriched fungus from natural environment and evaluation of organically bound iron bioavailability in rats |
title_full_unstemmed |
Screening of iron-enriched fungus from natural environment and evaluation of organically bound iron bioavailability in rats |
title_sort |
screening of iron-enriched fungus from natural environment and evaluation of organically bound iron bioavailability in rats |
publisher |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
series |
Food Science and Technology |
issn |
1678-457X |
publishDate |
2015-03-01 |
description |
Iron is an essential element for nearly all living organisms, and its deficiency is the most common form of malnutrition in the world. The organic forms of trace elements are considered more bioavailable than the inorganic forms. Although Saccharomyces cerevisiae can enrich metal elements and convert inorganic iron to organic species, its tolerability and transforming capacity are limited. The aim of this study was to screen higher biomass and other iron-enriched fungi strains besides Saccharomyces cerevisiae from the natural environment. A PDA medium containing 800 μg/mL iron was used for initial screening. Fifty strains that tolerated high iron concentration were isolated from the natural environment, and only one strain, No.BY1109, grew well at Fe (II) concentration of 10,000μg/ml. According to morphological characterization, 18S rDNA sequence analysis, and biophysical and biochemical characterization, the strain No.BY1109 was identified as Rhodotorula. The iron content of No.BY1109 (10 mg Fe/g dry cell) was determined using atomic absorption spectrometry. The results of distribution of iron in the cells showed that iron ion was mainly chelated in the cell walls and vacuoles. The bioavailability in rats confirmed that strain No.BY1109 had higher absorption efficiency than that of ferrous sulfate after single dose oral administration. The present study introduces new iron supplements, and it is a basis for finding new iron supplements from natural environment. |
topic |
iron-rich fungus screening and isolation identification cell-iron distribution bioavailability |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612015000100058&lng=en&tlng=en |
work_keys_str_mv |
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