Cas9 Nickase-Assisted RNA Repression Enables Stable and Efficient Manipulation of Essential Metabolic Genes in Clostridium cellulolyticum

Essential gene functions remain largely underexplored in bacteria. Clostridium cellulolyticum is a promising candidate for consolidated bioprocessing; however, its genetic manipulation to reduce the formation of less-valuable acetate is technically challenging due to the essentiality of acetate-prod...

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Bibliographic Details
Main Authors: Tao Xu, Yongchao Li, Zhili He, Joy D. Van Nostrand, Jizhong Zhou
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-09-01
Series:Frontiers in Microbiology
Subjects:
Online Access:http://journal.frontiersin.org/article/10.3389/fmicb.2017.01744/full