Probiotic Effects on Multispecies Biofilm Composition, Architecture, and Caries Activity <i>In Vitro</i>
While probiotics have been tested for their anti-caries effect in vitro and also clinically, there is a lack of understanding of their effects on complex dental biofilms. We assessed two probiotics, <i>Lactobacillus reuteri</i> and <i>Streptococcus oligofermentans</i>, on a c...
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doaj-f9b1982d1e7e418d9eea738ce18a1bd72020-11-25T03:21:41ZengMDPI AGMicroorganisms2076-26072020-08-0181272127210.3390/microorganisms8091272Probiotic Effects on Multispecies Biofilm Composition, Architecture, and Caries Activity <i>In Vitro</i>Zhihui Chen0Sebastian Schlafer1Gerd Göstemeyer2Falk Schwendicke3Department of Oral Diagnostics, Digital Health and Health Services Research, Charité-Universitätsmedizin, 14197 Berlin, GermanyDepartment of Dentistry and Oral Health, Aarhus University, 8000 Aarhus, DenmarkDepartment of Operative and Preventive Dentistry, Charité-Universitätsmedizin Berlin, 14197 Berlin, GermanyDepartment of Oral Diagnostics, Digital Health and Health Services Research, Charité-Universitätsmedizin, 14197 Berlin, GermanyWhile probiotics have been tested for their anti-caries effect in vitro and also clinically, there is a lack of understanding of their effects on complex dental biofilms. We assessed two probiotics, <i>Lactobacillus reuteri</i> and <i>Streptococcus oligofermentans</i>, on a continuous-cultured model containing <i>Streptococcus mutans</i>, <i>Lactobacillus rhamnosus</i> and <i>Actinomyces naeslundii</i>. Cariogenic biofilms were grown on bovine enamel specimens and daily challenged with <i>L. reuteri</i> or <i>S. oligofermentans</i> whole culture (LC/SC) or cell-free supernatant (LS/SS) or medium only (negative control, NC) (<i>n</i> = 21/group) for 10 days. Biofilm was assessed via counting colony-forming units, quantitative polymerase chain reaction, and fluorescence in situ hybridization. Caries activity was determined by pH measurements and by assessing mineral loss (ΔZ) using transverse microradiography. Both LC and SC significantly reduced total and strain-specific cariogenic bacterial numbers (<i>p</i> < 0.05). ΔZ was reduced in LC (mean ± SD: 1846.67 ± 317.89) and SC (3315.87 ± 617.30) compared to NC (4681.48 ± 495.18, <i>p</i> < 0.05). No significant reductions in bacterial numbers and ΔZ was induced by supernatants. Biofilm architecture was not considerably affected by probiotic applications. Viable probiotics <i>L. reuteri</i> and <i>S. oligofermentans</i>, but not their culture supernatants, could reduce the caries activity of multi-species biofilms <i>in vitro</i>.https://www.mdpi.com/2076-2607/8/9/1272biofilm modeldemineralizationdental cariesenameltransverse microradiography |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhihui Chen Sebastian Schlafer Gerd Göstemeyer Falk Schwendicke |
spellingShingle |
Zhihui Chen Sebastian Schlafer Gerd Göstemeyer Falk Schwendicke Probiotic Effects on Multispecies Biofilm Composition, Architecture, and Caries Activity <i>In Vitro</i> Microorganisms biofilm model demineralization dental caries enamel transverse microradiography |
author_facet |
Zhihui Chen Sebastian Schlafer Gerd Göstemeyer Falk Schwendicke |
author_sort |
Zhihui Chen |
title |
Probiotic Effects on Multispecies Biofilm Composition, Architecture, and Caries Activity <i>In Vitro</i> |
title_short |
Probiotic Effects on Multispecies Biofilm Composition, Architecture, and Caries Activity <i>In Vitro</i> |
title_full |
Probiotic Effects on Multispecies Biofilm Composition, Architecture, and Caries Activity <i>In Vitro</i> |
title_fullStr |
Probiotic Effects on Multispecies Biofilm Composition, Architecture, and Caries Activity <i>In Vitro</i> |
title_full_unstemmed |
Probiotic Effects on Multispecies Biofilm Composition, Architecture, and Caries Activity <i>In Vitro</i> |
title_sort |
probiotic effects on multispecies biofilm composition, architecture, and caries activity <i>in vitro</i> |
publisher |
MDPI AG |
series |
Microorganisms |
issn |
2076-2607 |
publishDate |
2020-08-01 |
description |
While probiotics have been tested for their anti-caries effect in vitro and also clinically, there is a lack of understanding of their effects on complex dental biofilms. We assessed two probiotics, <i>Lactobacillus reuteri</i> and <i>Streptococcus oligofermentans</i>, on a continuous-cultured model containing <i>Streptococcus mutans</i>, <i>Lactobacillus rhamnosus</i> and <i>Actinomyces naeslundii</i>. Cariogenic biofilms were grown on bovine enamel specimens and daily challenged with <i>L. reuteri</i> or <i>S. oligofermentans</i> whole culture (LC/SC) or cell-free supernatant (LS/SS) or medium only (negative control, NC) (<i>n</i> = 21/group) for 10 days. Biofilm was assessed via counting colony-forming units, quantitative polymerase chain reaction, and fluorescence in situ hybridization. Caries activity was determined by pH measurements and by assessing mineral loss (ΔZ) using transverse microradiography. Both LC and SC significantly reduced total and strain-specific cariogenic bacterial numbers (<i>p</i> < 0.05). ΔZ was reduced in LC (mean ± SD: 1846.67 ± 317.89) and SC (3315.87 ± 617.30) compared to NC (4681.48 ± 495.18, <i>p</i> < 0.05). No significant reductions in bacterial numbers and ΔZ was induced by supernatants. Biofilm architecture was not considerably affected by probiotic applications. Viable probiotics <i>L. reuteri</i> and <i>S. oligofermentans</i>, but not their culture supernatants, could reduce the caries activity of multi-species biofilms <i>in vitro</i>. |
topic |
biofilm model demineralization dental caries enamel transverse microradiography |
url |
https://www.mdpi.com/2076-2607/8/9/1272 |
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