A robust method of nuclei isolation for single-cell RNA sequencing of solid tissues from the plant genus Populus.

Single-cell transcriptome analysis has been extensively applied in humans and animal models to uncover gene expression heterogeneity between the different cell types of a tissue or an organ. It demonstrated its capability to discover key regulatory elements that determine cell fate during developmen...

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Bibliographic Details
Main Authors: Daniel Conde, Paolo M Triozzi, Kelly M Balmant, Andria L Doty, Mariza Miranda, Anthony Boullosa, Henry W Schmidt, Wendell J Pereira, Christopher Dervinis, Matias Kirst
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2021-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0251149