A novel MSCRAMM sub-family in Coagulase negative staphylococcal species
Coagulase negative staphylococci are important opportunistic pathogens. Staphylococcus epidermidis, a coagulase negative staphylococcus, is the third leading cause of nosocomial infections in the US. Surface proteins like Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs)...
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2016-04-01
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doaj-fd3d06c04e3940dfad8d971be81a270a2020-11-24T22:39:31ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-04-01710.3389/fmicb.2016.00540185419A novel MSCRAMM sub-family in Coagulase negative staphylococcal speciesSrishtee eArora0Anne-Catrin eUhlemann1Franklin eLowy2Magnus eHook3Institute of Biosciences and Technology, Texas A&M University Health Science CenterCollege of Physicians and Surgeons, Columbia UniversityCollege of Physicians and Surgeons, Columbia UniversityInstitute of Biosciences and Technology, Texas A&M University Health Science CenterCoagulase negative staphylococci are important opportunistic pathogens. Staphylococcus epidermidis, a coagulase negative staphylococcus, is the third leading cause of nosocomial infections in the US. Surface proteins like Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs) are major virulence factors of pathogenic gram positive bacteria. Here, we identified a new chimeric protein; SesJ in S. epidermidis, which represents a prototype of a new subfamily of MSCRAMMs. Structural predictions show that SesJ has structural features characteristic of a MSCRAMM along with a N-Terminal repeat region and an Aspartic acid containing repeat region, features that have not been previously observed in staphylococcal MSCRAMMs but have been found in other surface proteins from gram positive bacteria. We identified and analyzed structural homologs of SesJ in three other coagulase negative staphylococci. These homologs of SesJ have an identical structural organization but varying sequence identities within the domains. Using flow cytometry, we also show that SesJ is expressed constitutively on the surface of a representative S. epidermidis strain, from early exponential to stationary growth phase. Thus SesJ is positioned to interact with protein targets in the environment and play a role in S. epidermidis virulence.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00540/fullStaphylococcus epidermidisCoagulase negative StaphylococciMSCRAMMcell wall anchored proteinsstructural homologsN-terminal repeats |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Srishtee eArora Anne-Catrin eUhlemann Franklin eLowy Magnus eHook |
spellingShingle |
Srishtee eArora Anne-Catrin eUhlemann Franklin eLowy Magnus eHook A novel MSCRAMM sub-family in Coagulase negative staphylococcal species Frontiers in Microbiology Staphylococcus epidermidis Coagulase negative Staphylococci MSCRAMM cell wall anchored proteins structural homologs N-terminal repeats |
author_facet |
Srishtee eArora Anne-Catrin eUhlemann Franklin eLowy Magnus eHook |
author_sort |
Srishtee eArora |
title |
A novel MSCRAMM sub-family in Coagulase negative staphylococcal species |
title_short |
A novel MSCRAMM sub-family in Coagulase negative staphylococcal species |
title_full |
A novel MSCRAMM sub-family in Coagulase negative staphylococcal species |
title_fullStr |
A novel MSCRAMM sub-family in Coagulase negative staphylococcal species |
title_full_unstemmed |
A novel MSCRAMM sub-family in Coagulase negative staphylococcal species |
title_sort |
novel mscramm sub-family in coagulase negative staphylococcal species |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2016-04-01 |
description |
Coagulase negative staphylococci are important opportunistic pathogens. Staphylococcus epidermidis, a coagulase negative staphylococcus, is the third leading cause of nosocomial infections in the US. Surface proteins like Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs) are major virulence factors of pathogenic gram positive bacteria. Here, we identified a new chimeric protein; SesJ in S. epidermidis, which represents a prototype of a new subfamily of MSCRAMMs. Structural predictions show that SesJ has structural features characteristic of a MSCRAMM along with a N-Terminal repeat region and an Aspartic acid containing repeat region, features that have not been previously observed in staphylococcal MSCRAMMs but have been found in other surface proteins from gram positive bacteria. We identified and analyzed structural homologs of SesJ in three other coagulase negative staphylococci. These homologs of SesJ have an identical structural organization but varying sequence identities within the domains. Using flow cytometry, we also show that SesJ is expressed constitutively on the surface of a representative S. epidermidis strain, from early exponential to stationary growth phase. Thus SesJ is positioned to interact with protein targets in the environment and play a role in S. epidermidis virulence. |
topic |
Staphylococcus epidermidis Coagulase negative Staphylococci MSCRAMM cell wall anchored proteins structural homologs N-terminal repeats |
url |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00540/full |
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