Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis

Abstract Poly(ethylene terephthalate) (PET) is a widely used plastic in bottles and fibers; its waste products pollute the environment owing to its remarkable durability. Recently, Ideonella sakaiensis 201-F6 was isolated as a unique bacterium that can degrade and assimilate PET, thus paving the way...

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Main Authors: Ryoga Fujiwara, Rikako Sanuki, Hiroharu Ajiro, Toshiaki Fukui, Shosuke Yoshida
Format: Article
Language:English
Published: Nature Publishing Group 2021-10-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-99528-x
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spelling doaj-fd4f4c8c545d4a289f2064bb54d4685e2021-10-10T11:28:26ZengNature Publishing GroupScientific Reports2045-23222021-10-011111710.1038/s41598-021-99528-xDirect fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensisRyoga Fujiwara0Rikako Sanuki1Hiroharu Ajiro2Toshiaki Fukui3Shosuke Yoshida4Graduate School of Biological Science, Nara Institute of Science and TechnologyDepartment of Applied Biology, Kyoto Institute of TechnologyDivision of Materials Science, Graduate School of Science and Technology, Nara Institute of Science and TechnologySchool of Life Science and Technology, Tokyo Institute of TechnologyDivision for Research Strategy, Institute for Research Initiatives, Nara Institute of Science and TechnologyAbstract Poly(ethylene terephthalate) (PET) is a widely used plastic in bottles and fibers; its waste products pollute the environment owing to its remarkable durability. Recently, Ideonella sakaiensis 201-F6 was isolated as a unique bacterium that can degrade and assimilate PET, thus paving the way for the bioremediation and bioconversion of PET waste. We found that this strain harbors a poly(hydroxyalkanoate) (PHA) synthesis gene cluster, which is highly homologous with that of Cupriavidus necator, an efficient PHA producer. Cells grown on PET accumulated intracellular PHA at high levels. Collectively, our findings in this study demonstrate that I. sakaiensis can mediate the direct conversion of non-biodegradable PET into environment-friendly plastic, providing a new approach for PET recycling.https://doi.org/10.1038/s41598-021-99528-x
collection DOAJ
language English
format Article
sources DOAJ
author Ryoga Fujiwara
Rikako Sanuki
Hiroharu Ajiro
Toshiaki Fukui
Shosuke Yoshida
spellingShingle Ryoga Fujiwara
Rikako Sanuki
Hiroharu Ajiro
Toshiaki Fukui
Shosuke Yoshida
Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis
Scientific Reports
author_facet Ryoga Fujiwara
Rikako Sanuki
Hiroharu Ajiro
Toshiaki Fukui
Shosuke Yoshida
author_sort Ryoga Fujiwara
title Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis
title_short Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis
title_full Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis
title_fullStr Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis
title_full_unstemmed Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis
title_sort direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by ideonella sakaiensis
publisher Nature Publishing Group
series Scientific Reports
issn 2045-2322
publishDate 2021-10-01
description Abstract Poly(ethylene terephthalate) (PET) is a widely used plastic in bottles and fibers; its waste products pollute the environment owing to its remarkable durability. Recently, Ideonella sakaiensis 201-F6 was isolated as a unique bacterium that can degrade and assimilate PET, thus paving the way for the bioremediation and bioconversion of PET waste. We found that this strain harbors a poly(hydroxyalkanoate) (PHA) synthesis gene cluster, which is highly homologous with that of Cupriavidus necator, an efficient PHA producer. Cells grown on PET accumulated intracellular PHA at high levels. Collectively, our findings in this study demonstrate that I. sakaiensis can mediate the direct conversion of non-biodegradable PET into environment-friendly plastic, providing a new approach for PET recycling.
url https://doi.org/10.1038/s41598-021-99528-x
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