Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis
Abstract Poly(ethylene terephthalate) (PET) is a widely used plastic in bottles and fibers; its waste products pollute the environment owing to its remarkable durability. Recently, Ideonella sakaiensis 201-F6 was isolated as a unique bacterium that can degrade and assimilate PET, thus paving the way...
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2021-10-01
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Online Access: | https://doi.org/10.1038/s41598-021-99528-x |
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doaj-fd4f4c8c545d4a289f2064bb54d4685e2021-10-10T11:28:26ZengNature Publishing GroupScientific Reports2045-23222021-10-011111710.1038/s41598-021-99528-xDirect fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensisRyoga Fujiwara0Rikako Sanuki1Hiroharu Ajiro2Toshiaki Fukui3Shosuke Yoshida4Graduate School of Biological Science, Nara Institute of Science and TechnologyDepartment of Applied Biology, Kyoto Institute of TechnologyDivision of Materials Science, Graduate School of Science and Technology, Nara Institute of Science and TechnologySchool of Life Science and Technology, Tokyo Institute of TechnologyDivision for Research Strategy, Institute for Research Initiatives, Nara Institute of Science and TechnologyAbstract Poly(ethylene terephthalate) (PET) is a widely used plastic in bottles and fibers; its waste products pollute the environment owing to its remarkable durability. Recently, Ideonella sakaiensis 201-F6 was isolated as a unique bacterium that can degrade and assimilate PET, thus paving the way for the bioremediation and bioconversion of PET waste. We found that this strain harbors a poly(hydroxyalkanoate) (PHA) synthesis gene cluster, which is highly homologous with that of Cupriavidus necator, an efficient PHA producer. Cells grown on PET accumulated intracellular PHA at high levels. Collectively, our findings in this study demonstrate that I. sakaiensis can mediate the direct conversion of non-biodegradable PET into environment-friendly plastic, providing a new approach for PET recycling.https://doi.org/10.1038/s41598-021-99528-x |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ryoga Fujiwara Rikako Sanuki Hiroharu Ajiro Toshiaki Fukui Shosuke Yoshida |
spellingShingle |
Ryoga Fujiwara Rikako Sanuki Hiroharu Ajiro Toshiaki Fukui Shosuke Yoshida Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis Scientific Reports |
author_facet |
Ryoga Fujiwara Rikako Sanuki Hiroharu Ajiro Toshiaki Fukui Shosuke Yoshida |
author_sort |
Ryoga Fujiwara |
title |
Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis |
title_short |
Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis |
title_full |
Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis |
title_fullStr |
Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis |
title_full_unstemmed |
Direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by Ideonella sakaiensis |
title_sort |
direct fermentative conversion of poly(ethylene terephthalate) into poly(hydroxyalkanoate) by ideonella sakaiensis |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-10-01 |
description |
Abstract Poly(ethylene terephthalate) (PET) is a widely used plastic in bottles and fibers; its waste products pollute the environment owing to its remarkable durability. Recently, Ideonella sakaiensis 201-F6 was isolated as a unique bacterium that can degrade and assimilate PET, thus paving the way for the bioremediation and bioconversion of PET waste. We found that this strain harbors a poly(hydroxyalkanoate) (PHA) synthesis gene cluster, which is highly homologous with that of Cupriavidus necator, an efficient PHA producer. Cells grown on PET accumulated intracellular PHA at high levels. Collectively, our findings in this study demonstrate that I. sakaiensis can mediate the direct conversion of non-biodegradable PET into environment-friendly plastic, providing a new approach for PET recycling. |
url |
https://doi.org/10.1038/s41598-021-99528-x |
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