Variations in phyllosphere microbial community along with the development of angular leaf-spot of cucumber
Abstract The phyllosphere is colonized by a wide variety of microorganisms including epiphytes, plant-pathogenic fungus, bacteria, as well as human or animal pathogens. However, little is known about how microbial community composition changes with the development of angular leaf-spot of cucumber. H...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
SpringerOpen
2019-05-01
|
Series: | AMB Express |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s13568-019-0800-y |
id |
doaj-fde7820b43b54dce98a996472269386e |
---|---|
record_format |
Article |
spelling |
doaj-fde7820b43b54dce98a996472269386e2020-11-25T03:17:55ZengSpringerOpenAMB Express2191-08552019-05-019111310.1186/s13568-019-0800-yVariations in phyllosphere microbial community along with the development of angular leaf-spot of cucumberLuyun Luo0Zhuo Zhang1Pei Wang2Yongqin Han3Decai Jin4Pin Su5Xinqiu Tan6Deyong Zhang7Hamid Muhammad-Rizwan8Xiangyang Lu9Yong Liu10College of Bioscience & Biotechnology, Hunan Agricultural UniversityKey Laboratory of Pest Management of Horticultural Crops of Hunan Province, Hunan Plant Protection Institute, Hunan Academy of Agricultural ScienceKey Laboratory of Pest Management of Horticultural Crops of Hunan Province, Hunan Plant Protection Institute, Hunan Academy of Agricultural ScienceKey Laboratory of Pest Management of Horticultural Crops of Hunan Province, Hunan Plant Protection Institute, Hunan Academy of Agricultural ScienceChinese Academy of Sciences Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of SciencesKey Laboratory of Pest Management of Horticultural Crops of Hunan Province, Hunan Plant Protection Institute, Hunan Academy of Agricultural ScienceCollege of Bioscience & Biotechnology, Hunan Agricultural UniversityCollege of Bioscience & Biotechnology, Hunan Agricultural UniversityKey Laboratory of Pest Management of Horticultural Crops of Hunan Province, Hunan Plant Protection Institute, Hunan Academy of Agricultural ScienceCollege of Bioscience & Biotechnology, Hunan Agricultural UniversityKey Laboratory of Pest Management of Horticultural Crops of Hunan Province, Hunan Plant Protection Institute, Hunan Academy of Agricultural ScienceAbstract The phyllosphere is colonized by a wide variety of microorganisms including epiphytes, plant-pathogenic fungus, bacteria, as well as human or animal pathogens. However, little is known about how microbial community composition changes with the development of angular leaf-spot of cucumber. Here, 18 mixed samples were collected based on the lesion coverage rate (LCR) of angular leaf-spot of cucumber from three disease severity groups (DM1: symptomatic-mild, DM2: symptomatic-moderate, DM3: symptomatic-severe). In our study, the microbial community structure and diversity were examined by Illumina MiSeq sequencing. A significant differences was observed in α diversity and community structure among three disease severity groups. The phyllosphere microbiota was observed to be dominated by bacterial populations from Proteobacteria, Actinobacteria, and Firmicutes, as well as fungal species from Ascomycota and Basidiomycota. In addition, some plant-specific microbe such as Sphingomonas, Methylobacterium, Pseudomonas, and Alternaria showed significant changes in their relative abundance of population. The LCR was correlated negatively with Sphingomonas, Methylobacterium, Quadrisphaera, and Lactobacillus, whereas correlated positively with Pseudomonas and Kineococcus (p < 0.05). The LCR was negatively correlated with Alternaria and Arthrinium of the fungal communities (p < 0.05). Molecular ecological networks of the microbial communities were constructed to show the interactions among the OTUs. Our current results indicated that the competitive relationships among species were broken with the development of angular leaf-spot of cucumber. The microbial community composition changed over the development of angular leaf-spot of cucumber. The result of molecular ecological networks indicated that the overall bacterial community tends toward mutualism from the competition. The development of angular leaf-spot of cucumber affected the ecosystem functioning by disrupting the stability of the microbial community network. This work will help us to understand the host plant-specific microbial community structures and shows how these communities change throughout the development of angular leaf-spot of cucumber.http://link.springer.com/article/10.1186/s13568-019-0800-yPhyllosphereMicrobial communityLesion coverage rateα DiversityPlant-specific microbe |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Luyun Luo Zhuo Zhang Pei Wang Yongqin Han Decai Jin Pin Su Xinqiu Tan Deyong Zhang Hamid Muhammad-Rizwan Xiangyang Lu Yong Liu |
spellingShingle |
Luyun Luo Zhuo Zhang Pei Wang Yongqin Han Decai Jin Pin Su Xinqiu Tan Deyong Zhang Hamid Muhammad-Rizwan Xiangyang Lu Yong Liu Variations in phyllosphere microbial community along with the development of angular leaf-spot of cucumber AMB Express Phyllosphere Microbial community Lesion coverage rate α Diversity Plant-specific microbe |
author_facet |
Luyun Luo Zhuo Zhang Pei Wang Yongqin Han Decai Jin Pin Su Xinqiu Tan Deyong Zhang Hamid Muhammad-Rizwan Xiangyang Lu Yong Liu |
author_sort |
Luyun Luo |
title |
Variations in phyllosphere microbial community along with the development of angular leaf-spot of cucumber |
title_short |
Variations in phyllosphere microbial community along with the development of angular leaf-spot of cucumber |
title_full |
Variations in phyllosphere microbial community along with the development of angular leaf-spot of cucumber |
title_fullStr |
Variations in phyllosphere microbial community along with the development of angular leaf-spot of cucumber |
title_full_unstemmed |
Variations in phyllosphere microbial community along with the development of angular leaf-spot of cucumber |
title_sort |
variations in phyllosphere microbial community along with the development of angular leaf-spot of cucumber |
publisher |
SpringerOpen |
series |
AMB Express |
issn |
2191-0855 |
publishDate |
2019-05-01 |
description |
Abstract The phyllosphere is colonized by a wide variety of microorganisms including epiphytes, plant-pathogenic fungus, bacteria, as well as human or animal pathogens. However, little is known about how microbial community composition changes with the development of angular leaf-spot of cucumber. Here, 18 mixed samples were collected based on the lesion coverage rate (LCR) of angular leaf-spot of cucumber from three disease severity groups (DM1: symptomatic-mild, DM2: symptomatic-moderate, DM3: symptomatic-severe). In our study, the microbial community structure and diversity were examined by Illumina MiSeq sequencing. A significant differences was observed in α diversity and community structure among three disease severity groups. The phyllosphere microbiota was observed to be dominated by bacterial populations from Proteobacteria, Actinobacteria, and Firmicutes, as well as fungal species from Ascomycota and Basidiomycota. In addition, some plant-specific microbe such as Sphingomonas, Methylobacterium, Pseudomonas, and Alternaria showed significant changes in their relative abundance of population. The LCR was correlated negatively with Sphingomonas, Methylobacterium, Quadrisphaera, and Lactobacillus, whereas correlated positively with Pseudomonas and Kineococcus (p < 0.05). The LCR was negatively correlated with Alternaria and Arthrinium of the fungal communities (p < 0.05). Molecular ecological networks of the microbial communities were constructed to show the interactions among the OTUs. Our current results indicated that the competitive relationships among species were broken with the development of angular leaf-spot of cucumber. The microbial community composition changed over the development of angular leaf-spot of cucumber. The result of molecular ecological networks indicated that the overall bacterial community tends toward mutualism from the competition. The development of angular leaf-spot of cucumber affected the ecosystem functioning by disrupting the stability of the microbial community network. This work will help us to understand the host plant-specific microbial community structures and shows how these communities change throughout the development of angular leaf-spot of cucumber. |
topic |
Phyllosphere Microbial community Lesion coverage rate α Diversity Plant-specific microbe |
url |
http://link.springer.com/article/10.1186/s13568-019-0800-y |
work_keys_str_mv |
AT luyunluo variationsinphyllospheremicrobialcommunityalongwiththedevelopmentofangularleafspotofcucumber AT zhuozhang variationsinphyllospheremicrobialcommunityalongwiththedevelopmentofangularleafspotofcucumber AT peiwang variationsinphyllospheremicrobialcommunityalongwiththedevelopmentofangularleafspotofcucumber AT yongqinhan variationsinphyllospheremicrobialcommunityalongwiththedevelopmentofangularleafspotofcucumber AT decaijin variationsinphyllospheremicrobialcommunityalongwiththedevelopmentofangularleafspotofcucumber AT pinsu variationsinphyllospheremicrobialcommunityalongwiththedevelopmentofangularleafspotofcucumber AT xinqiutan variationsinphyllospheremicrobialcommunityalongwiththedevelopmentofangularleafspotofcucumber AT deyongzhang variationsinphyllospheremicrobialcommunityalongwiththedevelopmentofangularleafspotofcucumber AT hamidmuhammadrizwan variationsinphyllospheremicrobialcommunityalongwiththedevelopmentofangularleafspotofcucumber AT xiangyanglu variationsinphyllospheremicrobialcommunityalongwiththedevelopmentofangularleafspotofcucumber AT yongliu variationsinphyllospheremicrobialcommunityalongwiththedevelopmentofangularleafspotofcucumber |
_version_ |
1724629061243764736 |