Identification and quantification of virulence factors of enterotoxigenic Escherichia coli by high-resolution melting curve quantitative PCR

Identification and quantification of virulence factors of enterotoxigenic Escherichia coli by high-resolution melting curve quantitative PCR

Abstract Background Diagnosis of enterotoxigenic E. coli (ETEC) associated diarrhea is complicated by the diversity of E.coli virulence factors. This study developed a multiplex quantitative PCR assay based on high-resolution melting curves analysis (HRM-qPCR) to identify and quantify genes encoding...

Full description

Bibliographic Details
Main Authors: Weilan Wang, Ruurd T. Zijlstra, Michael G. Gänzle
Format: Article
Language:English
Published: BMC 2017-05-01
Series:BMC Microbiology
Subjects:
Virulence factors
ETEC
HRM-qPCR
Diarrhea
Fimbriae
Online Access:http://link.springer.com/article/10.1186/s12866-017-1023-5

Internet

http://link.springer.com/article/10.1186/s12866-017-1023-5

Similar Items