Synthetic Biomimetic Coenzymes and Alcohol Dehydrogenases for Asymmetric Catalysis
Redox reactions catalyzed by highly selective nicotinamide-dependent oxidoreductases are rising to prominence in industry. The cost of nicotinamide adenine dinucleotide coenzymes has led to the use of well-established elaborate regeneration systems and more recently alternative synthetic biomimetic...
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doaj-fee6e17a363448da8c8157a9a007f3e12020-11-24T21:15:58ZengMDPI AGCatalysts2073-43442019-02-019320710.3390/catal9030207catal9030207Synthetic Biomimetic Coenzymes and Alcohol Dehydrogenases for Asymmetric CatalysisLaia Josa-Culleré0Antti S. K. Lahdenperä1Aubert Ribaucourt2Georg T. Höfler3Serena Gargiulo4Yuan-Yang Liu5Jian-He Xu6Jennifer Cassidy7Francesca Paradisi8Diederik J. Opperman9Frank Hollmann10Caroline E. Paul11Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The NetherlandsDepartment of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The NetherlandsDepartment of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The NetherlandsDepartment of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The NetherlandsDepartment of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The NetherlandsState Key Laboratory of Bioreactor Engineering and Shanghai Collaborative Innovation Centre for Biomanufacturing, East China University of Science and Technology, Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering and Shanghai Collaborative Innovation Centre for Biomanufacturing, East China University of Science and Technology, Shanghai 200237, ChinaUniversity College Dublin, Belfield, Dublin 4, IrelandSchool of Chemistry, University of Nottingham, Nottingham NG7 2RD, UKDepartment of Biotechnology, University of the Free State, 205 Nelson Mandela Drive, Bloemfontein 9300, South AfricaDepartment of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The NetherlandsDepartment of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The NetherlandsRedox reactions catalyzed by highly selective nicotinamide-dependent oxidoreductases are rising to prominence in industry. The cost of nicotinamide adenine dinucleotide coenzymes has led to the use of well-established elaborate regeneration systems and more recently alternative synthetic biomimetic cofactors. These biomimetics are highly attractive to use with ketoreductases for asymmetric catalysis. In this work, we show that the commonly studied cofactor analogue 1-benzyl-1,4-dihydronicotinamide (BNAH) can be used with alcohol dehydrogenases (ADHs) under certain conditions. First, we carried out the rhodium-catalyzed recycling of BNAH with horse liver ADH (HLADH), observing enantioenriched product only with unpurified enzyme. Then, a series of cell-free extracts and purified ketoreductases were screened with BNAH. The use of unpurified enzyme led to product formation, whereas upon dialysis or further purification no product was observed. Several other biomimetics were screened with various ADHs and showed no or very low activity, but also no inhibition. BNAH as a hydride source was shown to directly reduce nicotinamide adenine dinucleotide (NAD) to NADH. A formate dehydrogenase could also mediate the reduction of NAD from BNAH. BNAH was established to show no or very low activity with ADHs and could be used as a hydride donor to recycle NADH.https://www.mdpi.com/2073-4344/9/3/207alcohol dehydrogenasescofactor regenerationformate dehydrogenasenicotinamide coenzyme biomimeticsrhodium catalyst |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Laia Josa-Culleré Antti S. K. Lahdenperä Aubert Ribaucourt Georg T. Höfler Serena Gargiulo Yuan-Yang Liu Jian-He Xu Jennifer Cassidy Francesca Paradisi Diederik J. Opperman Frank Hollmann Caroline E. Paul |
spellingShingle |
Laia Josa-Culleré Antti S. K. Lahdenperä Aubert Ribaucourt Georg T. Höfler Serena Gargiulo Yuan-Yang Liu Jian-He Xu Jennifer Cassidy Francesca Paradisi Diederik J. Opperman Frank Hollmann Caroline E. Paul Synthetic Biomimetic Coenzymes and Alcohol Dehydrogenases for Asymmetric Catalysis Catalysts alcohol dehydrogenases cofactor regeneration formate dehydrogenase nicotinamide coenzyme biomimetics rhodium catalyst |
author_facet |
Laia Josa-Culleré Antti S. K. Lahdenperä Aubert Ribaucourt Georg T. Höfler Serena Gargiulo Yuan-Yang Liu Jian-He Xu Jennifer Cassidy Francesca Paradisi Diederik J. Opperman Frank Hollmann Caroline E. Paul |
author_sort |
Laia Josa-Culleré |
title |
Synthetic Biomimetic Coenzymes and Alcohol Dehydrogenases for Asymmetric Catalysis |
title_short |
Synthetic Biomimetic Coenzymes and Alcohol Dehydrogenases for Asymmetric Catalysis |
title_full |
Synthetic Biomimetic Coenzymes and Alcohol Dehydrogenases for Asymmetric Catalysis |
title_fullStr |
Synthetic Biomimetic Coenzymes and Alcohol Dehydrogenases for Asymmetric Catalysis |
title_full_unstemmed |
Synthetic Biomimetic Coenzymes and Alcohol Dehydrogenases for Asymmetric Catalysis |
title_sort |
synthetic biomimetic coenzymes and alcohol dehydrogenases for asymmetric catalysis |
publisher |
MDPI AG |
series |
Catalysts |
issn |
2073-4344 |
publishDate |
2019-02-01 |
description |
Redox reactions catalyzed by highly selective nicotinamide-dependent oxidoreductases are rising to prominence in industry. The cost of nicotinamide adenine dinucleotide coenzymes has led to the use of well-established elaborate regeneration systems and more recently alternative synthetic biomimetic cofactors. These biomimetics are highly attractive to use with ketoreductases for asymmetric catalysis. In this work, we show that the commonly studied cofactor analogue 1-benzyl-1,4-dihydronicotinamide (BNAH) can be used with alcohol dehydrogenases (ADHs) under certain conditions. First, we carried out the rhodium-catalyzed recycling of BNAH with horse liver ADH (HLADH), observing enantioenriched product only with unpurified enzyme. Then, a series of cell-free extracts and purified ketoreductases were screened with BNAH. The use of unpurified enzyme led to product formation, whereas upon dialysis or further purification no product was observed. Several other biomimetics were screened with various ADHs and showed no or very low activity, but also no inhibition. BNAH as a hydride source was shown to directly reduce nicotinamide adenine dinucleotide (NAD) to NADH. A formate dehydrogenase could also mediate the reduction of NAD from BNAH. BNAH was established to show no or very low activity with ADHs and could be used as a hydride donor to recycle NADH. |
topic |
alcohol dehydrogenases cofactor regeneration formate dehydrogenase nicotinamide coenzyme biomimetics rhodium catalyst |
url |
https://www.mdpi.com/2073-4344/9/3/207 |
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