Expression and purification of ELP-intein-tagged target proteins in high cell density <it>E. coli </it>fermentation

<p>Abstract</p> <p>Background</p> <p>Elastin-like polypeptides (ELPs) are useful tools that can be used to non-chromatographically purify proteins. When paired with self-cleaving inteins, they can be used as economical self-cleaving purification tags. However, ELPs and...

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Bibliographic Details
Main Authors: Wood David W, Fong Baley A
Format: Article
Language:English
Published: BMC 2010-10-01
Series:Microbial Cell Factories
Online Access:http://www.microbialcellfactories.com/content/9/1/77