Expression and purification of ELP-intein-tagged target proteins in high cell density <it>E. coli </it>fermentation
<p>Abstract</p> <p>Background</p> <p>Elastin-like polypeptides (ELPs) are useful tools that can be used to non-chromatographically purify proteins. When paired with self-cleaving inteins, they can be used as economical self-cleaving purification tags. However, ELPs and...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
BMC
2010-10-01
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Series: | Microbial Cell Factories |
Online Access: | http://www.microbialcellfactories.com/content/9/1/77 |