Engineering E. coli for the biosynthesis of 3-hydroxy-γ-butyrolactone (3HBL) and 3,4-dihydroxybutyric acid (3,4-DHBA) as value-added chemicals from glucose as a sole carbon source

3-hydroxy-γ-butyrolactone (3HBL) is a versatile chiral synthon, deemed a top value-added chemical from biomass by the DOE. We recently reported the first biosynthetic pathway towards 3HBL and its hydrolyzed form, 3,4-dihydroxybutyric acid (3,4-DHBA) in recombinant Escherichia coli using glucose and...

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Bibliographic Details
Main Authors: Tarasova, Yekaterina (Contributor), Martin, Collin H. (Contributor), Dhamankar, Himanshu Hemant (Author), Prather, Kristala L (Author)
Other Authors: Massachusetts Institute of Technology. Department of Biology (Contributor), Massachusetts Institute of Technology. Department of Chemical Engineering (Contributor), Massachusetts Institute of Technology. Microbiology Graduate Program (Contributor), Prather, Kristala L. Jones (Contributor), Dhamankar, Himanshu (Contributor)
Format: Article
Language:English
Published: Elsevier, 2016-03-01T20:39:43Z.
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Online Access:Get fulltext
LEADER 02549 am a22003013u 4500
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042 |a dc 
100 1 0 |a Tarasova, Yekaterina  |e author 
100 1 0 |a Massachusetts Institute of Technology. Department of Biology  |e contributor 
100 1 0 |a Massachusetts Institute of Technology. Department of Chemical Engineering  |e contributor 
100 1 0 |a Massachusetts Institute of Technology. Microbiology Graduate Program  |e contributor 
100 1 0 |a Prather, Kristala L. Jones  |e contributor 
100 1 0 |a Dhamankar, Himanshu  |e contributor 
100 1 0 |a Tarasova, Yekaterina  |e contributor 
100 1 0 |a Martin, Collin H.  |e contributor 
100 1 0 |a Prather, Kristala L. Jones  |e contributor 
700 1 0 |a Martin, Collin H.  |e author 
700 1 0 |a Dhamankar, Himanshu Hemant  |e author 
700 1 0 |a Prather, Kristala L  |e author 
245 0 0 |a Engineering E. coli for the biosynthesis of 3-hydroxy-γ-butyrolactone (3HBL) and 3,4-dihydroxybutyric acid (3,4-DHBA) as value-added chemicals from glucose as a sole carbon source 
260 |b Elsevier,   |c 2016-03-01T20:39:43Z. 
856 |z Get fulltext  |u http://hdl.handle.net/1721.1/101387 
520 |a 3-hydroxy-γ-butyrolactone (3HBL) is a versatile chiral synthon, deemed a top value-added chemical from biomass by the DOE. We recently reported the first biosynthetic pathway towards 3HBL and its hydrolyzed form, 3,4-dihydroxybutyric acid (3,4-DHBA) in recombinant Escherichia coli using glucose and glycolic acid as feedstocks and briefly described their synthesis solely from glucose. Synthesis from glucose requires integration of the endogenous glyoxylate shunt with the 3,4-DHBA/3HBL pathway and co-overexpression of seven genes, posing challenges with respect to expression, repression of the glyoxylate shunt and optimal carbon distribution between the two pathways. Here we discuss engineering this integration. While appropriate media and over-expression of glyoxylate shunt enzymes helped overcome repression, two orthogonal expression systems were employed to address the expression and carbon distribution challenge. Synthesis of up to 0.3 g/L of 3HBL and 0.7 g/L of 3,4-DHBA solely from glucose was demonstrated, amounting to 24% of the theoretical maximum. 
520 |a National Science Foundation (U.S.). Synthetic Biology Engineering Research Center (Grant EEC-0540879) 
520 |a Masdar Institute of Science and Technology (Massachusetts Institute of Technology Cooperative Agreement 02/MI/MI/CP/11/07633/GEN/G/00) 
546 |a en_US 
655 7 |a Article 
773 |t Metabolic Engineering