Scarless Cas9 Assisted Recombineering (no‐SCAR) in Escherichia coli, an Easy‐to‐Use System for Genome Editing

The discovery and development of genome editing systems that leverage the site‐specific DNA endonuclease system CRISPR/Cas9 has fundamentally changed the ease and speed of genome editing in many organisms. In eukaryotes, the CRISPR/Cas9 system utilizes a "guide" RNA to enable the Cas9 nucl...

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Bibliographic Details
Main Authors: Reisch, Christopher R (Contributor), Jones, Kristala L. (Contributor)
Other Authors: Massachusetts Institute of Technology. Department of Chemical Engineering (Contributor), Prather, Kristala L. Jones (Contributor)
Format: Article
Published: Wiley Blackwell, 2018-05-01T16:44:42Z.
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