Scarless Cas9 Assisted Recombineering (no‐SCAR) in Escherichia coli, an Easy‐to‐Use System for Genome Editing
The discovery and development of genome editing systems that leverage the site‐specific DNA endonuclease system CRISPR/Cas9 has fundamentally changed the ease and speed of genome editing in many organisms. In eukaryotes, the CRISPR/Cas9 system utilizes a "guide" RNA to enable the Cas9 nucl...
Main Authors: | , |
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Other Authors: | , |
Format: | Article |
Language: | English |
Published: |
Wiley Blackwell,
2018-05-01T16:44:42Z.
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Subjects: | |
Online Access: | Get fulltext |