Fabrication of microfluidic devices for artificial respiration

Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2007. === Includes bibliographical references (p. 101-108). === We are developing elastomeric polydimethylsiloxane (PDMS) microfluidic devices incorporated with photoactive thin films to create an implantable arti...

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Main Author: Park, Hyesung, Ph. D. Massachusetts Institute of Technology
Other Authors: Todd Thorsen and Richard Gilbert.
Format: Others
Language:English
Published: Massachusetts Institute of Technology 2008
Subjects:
Online Access:http://hdl.handle.net/1721.1/40370
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spelling ndltd-MIT-oai-dspace.mit.edu-1721.1-403702021-07-08T05:08:27Z Fabrication of microfluidic devices for artificial respiration Park, Hyesung, Ph. D. Massachusetts Institute of Technology Todd Thorsen and Richard Gilbert. Massachusetts Institute of Technology. Dept. of Mechanical Engineering. Massachusetts Institute of Technology. Dept. of Mechanical Engineering. Massachusetts Institute of Technology. Department of Mechanical Engineering Mechanical Engineering. Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2007. Includes bibliographical references (p. 101-108). We are developing elastomeric polydimethylsiloxane (PDMS) microfluidic devices incorporated with photoactive thin films to create an implantable artificial respiration platform. Whereas state-of-the-art respiration support machines deliver oxygen gas directly to the blood via external macroscale devices, our technique utilizes a biomimetic photocatalytic process to generate energy from light and thus produce dissolved oxygen from water which is already present in the blood. Blood oxygenation will be achieved by the interaction between the photoactivated metal oxide film and blood in the setting of a molded microfluidic conduit, providing a stable and implantable oxygenation platform. As a basic, scalable building block, we developed a noble "network" design which was structurally similar to the native pulmonary capillary network. The interconnected channel geometry was designed in such a way to minimize shear stress and reduce hemolysis and thrombosis inside the microchannel. It allowed alternative flow pathways in the event of single channel occlusion while minimizing the establishment of detrimental pressure gradients. The hemocompatibility analysis demonstrated that the network construct showed acceptable levels of hemolysis rate (< 8%) and thrombus formation. (cont.) Critical to the success of this project is the understanding of the manufacture parameters for microfluidic devices molded from elastomeric materials like PDMS. In the initial development of our work, we performed the following three tasks to generate manufacture protocols for elastomeric microfluidic devices that will be ultimately used for biological applications: 1) Curing schedules of the heat-cure PDMS elastomers under various fabrication parameters were characterized. 2) The interlayer bonding chemistry of the double layer PDMS device was analyzed followed by subsequent mechanical analysis. 3) The efficacy of various surface treatment techniques on hydrophobic PDMS surfaces was investigated using fluorescently tagged bacteria (E. Coli) flowed through microchannels as reporter particles to measure non-specific adhesion, which will provide useful information in minimizing channel fouling for biological applications. by Hyesung Park. S.M. 2008-02-27T22:15:22Z 2008-02-27T22:15:22Z 2007 2007 Thesis http://hdl.handle.net/1721.1/40370 190863773 eng M.I.T. theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. See provided URL for inquiries about permission. http://dspace.mit.edu/handle/1721.1/7582 108 p. application/pdf Massachusetts Institute of Technology
collection NDLTD
language English
format Others
sources NDLTD
topic Mechanical Engineering.
spellingShingle Mechanical Engineering.
Park, Hyesung, Ph. D. Massachusetts Institute of Technology
Fabrication of microfluidic devices for artificial respiration
description Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2007. === Includes bibliographical references (p. 101-108). === We are developing elastomeric polydimethylsiloxane (PDMS) microfluidic devices incorporated with photoactive thin films to create an implantable artificial respiration platform. Whereas state-of-the-art respiration support machines deliver oxygen gas directly to the blood via external macroscale devices, our technique utilizes a biomimetic photocatalytic process to generate energy from light and thus produce dissolved oxygen from water which is already present in the blood. Blood oxygenation will be achieved by the interaction between the photoactivated metal oxide film and blood in the setting of a molded microfluidic conduit, providing a stable and implantable oxygenation platform. As a basic, scalable building block, we developed a noble "network" design which was structurally similar to the native pulmonary capillary network. The interconnected channel geometry was designed in such a way to minimize shear stress and reduce hemolysis and thrombosis inside the microchannel. It allowed alternative flow pathways in the event of single channel occlusion while minimizing the establishment of detrimental pressure gradients. The hemocompatibility analysis demonstrated that the network construct showed acceptable levels of hemolysis rate (< 8%) and thrombus formation. === (cont.) Critical to the success of this project is the understanding of the manufacture parameters for microfluidic devices molded from elastomeric materials like PDMS. In the initial development of our work, we performed the following three tasks to generate manufacture protocols for elastomeric microfluidic devices that will be ultimately used for biological applications: 1) Curing schedules of the heat-cure PDMS elastomers under various fabrication parameters were characterized. 2) The interlayer bonding chemistry of the double layer PDMS device was analyzed followed by subsequent mechanical analysis. 3) The efficacy of various surface treatment techniques on hydrophobic PDMS surfaces was investigated using fluorescently tagged bacteria (E. Coli) flowed through microchannels as reporter particles to measure non-specific adhesion, which will provide useful information in minimizing channel fouling for biological applications. === by Hyesung Park. === S.M.
author2 Todd Thorsen and Richard Gilbert.
author_facet Todd Thorsen and Richard Gilbert.
Park, Hyesung, Ph. D. Massachusetts Institute of Technology
author Park, Hyesung, Ph. D. Massachusetts Institute of Technology
author_sort Park, Hyesung, Ph. D. Massachusetts Institute of Technology
title Fabrication of microfluidic devices for artificial respiration
title_short Fabrication of microfluidic devices for artificial respiration
title_full Fabrication of microfluidic devices for artificial respiration
title_fullStr Fabrication of microfluidic devices for artificial respiration
title_full_unstemmed Fabrication of microfluidic devices for artificial respiration
title_sort fabrication of microfluidic devices for artificial respiration
publisher Massachusetts Institute of Technology
publishDate 2008
url http://hdl.handle.net/1721.1/40370
work_keys_str_mv AT parkhyesungphdmassachusettsinstituteoftechnology fabricationofmicrofluidicdevicesforartificialrespiration
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