Bioaugmentation for the remediation of pesticide-contaminated soil with microorganisms directly enriched in soil or compost

Bibliographic Details
Main Author: Kim, Sang-Jun
Language:English
Published: The Ohio State University / OhioLINK 2003
Subjects:
Online Access:http://rave.ohiolink.edu/etdc/view?acc_num=osu1062640058
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spelling ndltd-OhioLink-oai-etd.ohiolink.edu-osu10626400582021-08-03T05:48:25Z Bioaugmentation for the remediation of pesticide-contaminated soil with microorganisms directly enriched in soil or compost Kim, Sang-Jun Bioaugmentation Atrazine EPTC Enrichment Bioremediation Pesticide EPTC (s-ethyl-N,N’-dipropylthiocarbamate) and atrazine (2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine) are commonly used herbicides. Atrazine, especially, can be found in soils and waters at unacceptable concentrations. Developing an effective inoculum to bioaugment degradation of EPTC and atrazine in contaminated environments is required for bioremediation of such sites. Microorganisms capable of EPTC or atrazine degradation were directly enriched in soils (hereafter called ACTIVATED soils). The most probable number method revealed that the number of EPTC-degraders in a Brookston soil increased by about 3 logs of magnitude after a single treatment with EPTC at a rate of 20 mg/kg soil. In a Wooster soil, microorganisms utilizing atrazine as their sole carbon or nitrogen source increased by 3 logs and 1 log of magnitude, respectively, after three treatments of atrazine at a rate of 4 mg/kg soil. EPTC or atrazine degradation in these ACTIVATED soils was greatly accelerated after the first treatment, and degradation activity was not inhibited at initial concentrations up to 2000 mg EPTC/kg and 400 mg atrazine/kg soil, respectively. Inoculation of EPTC (20 mg/kg)- or atrazine (4 mg/kg)-contaminated soils with ACTIVATED soil, at rates ranging from 0.05 to 5% (w/w), resulted in significantly (p < 0.05) increased degradation of EPTC or atrazine in four soils tested, compared to non-inoculated soil. Nutrient (glycine, sucrose, urea, or yeast extract) amendment at a 1% and 0.1% for EPTC- and atrazine-contaminated soils, respectively, did not further accelerate degradation. Both atrazine-degrading and mineralizing microbial populations were successfully transferred to and enriched in compost. The degradation activity in ACTIVATED compost was much greater than in ACTIVATED soil. The amount of atrazine dropped to about 10% of that initially present (about 400 mg/kg) after 9 hours of incubation in ACTIVATED compost, compared to 3 days in ACTIVATED soil. EPTC and atrazine degradation activity in ACTIVATED soil and compost remained stable when they were stored at low temperature (below 10 OC) and in a wet state (water content above 15%, w/w). ACTIVATED soil and compost, containing increased numbers of EPTC- or atrazine-degrading microorganisms, are useful inoculants that will bioaugment the remediation of EPTC or atrazine contaminated soil. 2003-11-07 English text The Ohio State University / OhioLINK http://rave.ohiolink.edu/etdc/view?acc_num=osu1062640058 http://rave.ohiolink.edu/etdc/view?acc_num=osu1062640058 unrestricted This thesis or dissertation is protected by copyright: all rights reserved. It may not be copied or redistributed beyond the terms of applicable copyright laws.
collection NDLTD
language English
sources NDLTD
topic Bioaugmentation
Atrazine
EPTC
Enrichment
Bioremediation
Pesticide
spellingShingle Bioaugmentation
Atrazine
EPTC
Enrichment
Bioremediation
Pesticide
Kim, Sang-Jun
Bioaugmentation for the remediation of pesticide-contaminated soil with microorganisms directly enriched in soil or compost
author Kim, Sang-Jun
author_facet Kim, Sang-Jun
author_sort Kim, Sang-Jun
title Bioaugmentation for the remediation of pesticide-contaminated soil with microorganisms directly enriched in soil or compost
title_short Bioaugmentation for the remediation of pesticide-contaminated soil with microorganisms directly enriched in soil or compost
title_full Bioaugmentation for the remediation of pesticide-contaminated soil with microorganisms directly enriched in soil or compost
title_fullStr Bioaugmentation for the remediation of pesticide-contaminated soil with microorganisms directly enriched in soil or compost
title_full_unstemmed Bioaugmentation for the remediation of pesticide-contaminated soil with microorganisms directly enriched in soil or compost
title_sort bioaugmentation for the remediation of pesticide-contaminated soil with microorganisms directly enriched in soil or compost
publisher The Ohio State University / OhioLINK
publishDate 2003
url http://rave.ohiolink.edu/etdc/view?acc_num=osu1062640058
work_keys_str_mv AT kimsangjun bioaugmentationfortheremediationofpesticidecontaminatedsoilwithmicroorganismsdirectlyenrichedinsoilorcompost
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