Production of oxytetracycline from immobilized streptomyces rimosus TM-55
碩士 === 國立臺灣大學 === 農業化學研究所 === 83 === Mycelia of Streptomyces rimosus TM-55 were immobilized in 2%(w/v) calcium-alginate at room temperature and used to produce oxyttetracycline in submerged fermentation.The maximum oxytetra- cycline production of free and immobi...
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| Format: | Others |
| Language: | zh-TW |
| Published: |
1995
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| Online Access: | http://ndltd.ncl.edu.tw/handle/38847634469317665246 |
| Summary: | 碩士 === 國立臺灣大學 === 農業化學研究所 === 83 === Mycelia of Streptomyces rimosus TM-55 were immobilized in 2%(w/v) calcium-alginate at room temperature and used to produce
oxyttetracycline in submerged fermentation.The maximum oxytetra-
cycline production of free and immobilized cells in submerged
fermentation was 124 and 153 ug/ml respectively.The relative
oxytetracycline productivity of immobilized cells was 124%
compared to that of free cells. Immobilization treatment
prolonged the growth of S. rimosus TM-55 and delayed its
stationary phase. The specific oxytetracycline productivity of
free and immobilized cells was 34.2 and 40.7mg/g dry cell weight
respectively. The optimal alginate concentration was 2 % (w/v).
smaller beads and higher inoculum density had greater oxytetra-
cycline production. EDTA (ethylenediamine tetra acetic acid) or
monovalent ions effected the beads structure and expanded or
even resolved the beads. Oxytetracycline could be absorbed and
released by the beads.Immobilization possesses great potential
in the industrial oxytetracycline production process. Further
physiological studies of immobilized cells and optimizaiton of
immobilizing condition will help to explore the application of
immobilization.
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