The Pharmacokinetics of Sarafloxacin in Cultured Eel(Anguilla anguilla)

• Main Authors: Cheng, Chin-Fu, 鄭清福
• Other Authors: Wang Way-Shyan
• Format: Others
• Language: zh-TW
• Published: 1996
• Online Access: http://ndltd.ncl.edu.tw/handle/07904782331031401406

碩士 === 國立中興大學 === 獸醫學系 === 84 === The aim of this study is to develop a simple, rapid, and reliable high per-formance liquid chromatography (HPLC) method for the simultaneous determinationof the first generation of quinolone (flumequine, nalidixic acid, and oxolinic acid) and the second generation of quinolones (enrofloxacin and sarafloxacin) in eel's meat. Based on the developed method, the single dose pharmacokineticsof sarafloxacin in eel (Anguilla anguilla) was studied. The simultaneous determination of five above quinolones in eel's meat was studied by a HPLC method. Quinolones were extracted with distilled water-acetonitrile (1:4). After homogenization of fish tissues, the fat separated byextraction into organic solvents and the aqueous phase was filtered than 20 ulaliquot of the filtrate were used for HPLC analysis. The separations were per-formed on LiChrospher RP-18 columns (150 * 4.6 mm I. D.) and guard columns with 0.01 M oxalic acid solution-acetonitrile-methanol-tetrahydrofuran (7.5:1.5:0.5:0.5, pH=3.0) as the mobile phase. A UV-VIS detector was used at a 260nm.The calibration graphs were linear from 1 ng to 200 ng for tested quinolones.The recoveries and detection limitation of the drug from tissues fortified at alevel of 1ug/g were 68.3%-84.9%and 0.07-0.5ug/g,respectively.The detection time of per sample was less than 40 min. On the determination of sarafloxacin in eel's tissues(liver,kindey,muscle,and skin)and plasms were detected in this investigation.The residues of sarafloxacin in fish tissues were extracted by the above simultaneous determination method; otherwise,the tissue's aqueous phase extract solution andthe plasma sample were loaded onto the C2 sorbent cartidge.Methanol-0.01M oxalic acid(7:3) was used for eluting sarafloxacin from the cartidge then the elution was determined by HPLC.A UV-VIS detector was used at a 278 nm.Thecalibration graph was linear from 1-500ng.The recoveries of the drug from tissues and plasma at a level of 1 ug/g(ml) were 73.4-91.1%.The detection limits were 0.07ug/g(ml) in all tissues and plasma. The pharmacokinetics of sarafloxacin in eel after single oral administration at a dose of 15mg/kg b. w. at 24C.The area under concertration-time curve(AUC)in plasma, liver,kidney,muscle,and skin was 12h,12h,12h,24h,and 40h.The peak concentration(Cmax) at Tmax in plasma,liver,kidney,muscle, and skinwas 2.64,13.39,5.53,1.82,and 0.78 ug/g(ml),respectively. On withdrawal of the drug,sarafloxacin concentration in plasma, liver,and kidney declined rapidly on the 28th,32nd,and 32nd h, respectively.It was conformed to be a biomodal elimination pattern with a rapid initial phase anda slower secondary phase. The distribution rate constant(a) for the line of best fit through the initial rapid phase(sample time 12th-32nd h) in plasma was 0.085h/l,(sample time 12th-28th h) liver and kidney were 0,12 and 0.071h/l,respectively.The distribution half-life( t1/2a)in plasma,liver,and kidney was 8.15,5.78,and 9.76 h, respectively.The elimination rate constant(B) for the lineof best fit through the slower final elimination phase and the elimination half-life(t1/2b) in plasma(sample time 32nd-96th h), liver,kidney(sample time28th-230th h),and skin(sample time 40th-240th h) was(0.023 h/l,30.13 h),(0,007 h/l,99 h),(0.012 h/ l,57.75h),(0.039 h/l,17.77 h),and(0.016 h/l,43.3 h), respectively. Sarafloxacin tissue levels below detectable limits in the plasma and muscleis 7days but liver,kidney, and skin is 14days.Based on the results of this study,we suggest that the best withdrawal period of sarafloxacin is 16 days.