| Summary: | 碩士 === 國立中興大學 === 食品科學系 === 85 === Abstract The objectives of this study were to investigate the
effects of various concentractions and incubation time intervals
of angelicin and psoralen, which are the active principles in
psoralea fruit (Psoralea corylifolia L.), on cell viability,
lipid peroxidation, antioxidation and detoxification
capabilities in rat hepatocytes. Primary rat hepatocytes were
used as experimental model and to examine the AFB1-induced DNA
damage of Chinese hamster ovary-K1 cells. From petroleum ether-
extrected psoralea fruit oleoresins two main principles were
separated using spectrophotometer, mass and 1H-NMR to appraise
the structures. The results confirmed that the two main
priciples were angelicin and psoralen with a purity of 97 % and
98 %, and individual yield of 0.38 % and 0.57 %, respectively.
According to the results of lactate dehydrogenase (LDH) leakage
and microscopic examination, 0.05 or 0.1 mM angelicin treatment
and 0.05, 0.1 or 0.5 mM psoralen treatment did not have
significant differences in affecting the viability of
hepatocytes after 4, 8, 24 and 48 hours of incubation. In other
words, 0.05 or 0.1 mM angelicin treatment did not cause any
damage of the hepatocytes. However,treatment with higher
concentractions of angelicin (0.5, 1 mM)and psoralen (1 mM),
significant increases in LDH l When hepatocytes were treated
with 0.1, 0.5 or 1 mM angelicin, the thiobarbituric acid-reative
substances (TBARS) value of hepatocytes was significantly
decreased (P<0.05). However, when treated with 0.05, 0.1, 0.5 or
1 mM psoralen, it did not cause lipid peroxidation. With regard
to the glutathione contents and its related enzyme activities,
higher levels of glutathione were obtained with 0.1 mM angelicin
treatment, and no adverse effects of glutathione S-transferase
(GST), glutathione peroxidase (GPx) and glutathione reductase
(GRd) activities. When hepatocytes were treated with 0.5 mM
psoralen, intracellular GSH level were higher than that in the
control (P<0.05), and the activities of GST, GPx and GRd were
also elevated (P<0.05). This phenomenon is beneificial to de
According to the results of sister chromatid exchanges (SCE),
angelicin and psoralen have dose-dependent decreased AFB1-
induced sister chromatid exchanges in CHO-K1 cells. The results
implied that angelicin and psoralen from psoralea fruit inhibit
genetic toxicity induced by AFB1.
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