| Summary: | 碩士 === 大同工學院 === 化學工程研究所 === 86 === A transglucosidase (TGase) of Aspergillus niger CCRC 31494 can catalyse bothnydrolytic and transfer reactions on incubation with isomalto-oligosaccharides.TGase can transfer the non-reducing D-glucosyl residue to water (hydrolysis), or to the non-reducing residue producing isomaltose from glucose or panose(6-O-a-D-glucosylmaltose) from maltose. It can also transfer the non-reducing D-glucosyl residue to C4-OH position of maltose and produce maltotriose. The enzyne is stable in storage at 40 C at least 9 hours. In organic solvent, ethanol,1-propanol, 2-propanol, and hexane can enhance hydrolysis about 120%. The activityis competitively inhibited by glucose and mannose, but is not ingibited by galactose and fructose. The Kis of glucose and mannose are 12.9 mM and 75.9 mM,respectively.Using maltose as substrate, the transglucosylation products were analyzed withHPLC. Based on product analysis and the kinetic properties of TGase. A suitablekinetic modle for illustrating the synthesis of isomaltooligosaccharides was deduced. The practical data were coincident to the deduced result.
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