Study of Signal Transduction of Epstein-Barr Virus EBNA-1 Protein in Human Epithelial Cell Lines
碩士 === 國立臺灣大學 === 微生物學研究所 === 87 === In Epstein-Barr virus (EBV) latently infected cells, EBNA-1 proteins could bind to the specific DNA sequences as a homodimer form in order to promote viral DNA replication and to maintain the viral DNA as an episomal form. Both lines of evidence that E...
Main Authors: | , |
---|---|
Other Authors: | |
Format: | Others |
Language: | zh-TW |
Published: |
1999
|
Online Access: | http://ndltd.ncl.edu.tw/handle/06560713075323897358 |
id |
ndltd-TW-087NTU01381020 |
---|---|
record_format |
oai_dc |
spelling |
ndltd-TW-087NTU013810202016-02-01T04:12:42Z http://ndltd.ncl.edu.tw/handle/06560713075323897358 Study of Signal Transduction of Epstein-Barr Virus EBNA-1 Protein in Human Epithelial Cell Lines EB病毒EBNA-1蛋白質在人類上皮細胞株訊號傳遞之研究 Liu Yu-sheng 劉育昇 碩士 國立臺灣大學 微生物學研究所 87 In Epstein-Barr virus (EBV) latently infected cells, EBNA-1 proteins could bind to the specific DNA sequences as a homodimer form in order to promote viral DNA replication and to maintain the viral DNA as an episomal form. Both lines of evidence that EBNA-1 protein was only viral protein existed in most EBV-associated malignancies and that EBNA-1 could induce B lymphoma in transgenic mice model, indicated that EBNA-1 might play some roles in the carcinogenesis of EBV-associated tumors. However, it is still not clear what cellular factors would be involved or regulated in this EBNA-1-mediated pathway. The major goal of this thesis was to investigate whatever cellular molecules regulated or activated by EBNA-1 using EBNA-1-expression stable clone and transient transfection assay system. In the preliminary results, it was observed that EBNA-1 could enhance the phosphorylation of p38 protein and increase the expression of Egr-1 in both stable and transient EBNA-1-expression RHEK-1 and 293 cells. Furthermore, the induction of Egr-1 protein by EBNA-1 could be suppressed by SB202190, a specific inhibitor for p38 phosphorylation. Both data suggested that EBNA-1 could stimulate the synthesis of Egr-1 protein through the action of phosphorylation of p38. It has been reported that NF-κB is one of the downstream molecule of p38 protein. According to the analysis in our luciferase activity assay, the activity of NF-κB could be increased at a dose-dependent manner by the presence of EBNA-1 proteins in the transfection assay. However, it still needs more experiments to prove whether this induction of NF-κB by EBNA-1 is mediated through p38 or not. We had found that the EBNA-1-expression RHEK-1 cells were grown better than the control RHEK-1 cells, in low serum culture condition. Here, the results of luciferase activity assay showed that both AP-1 and CRE activity were higher in the EBNA-1 expression cells at the low serum culture condition. We hypothesize that EBNA-1 could promote the cell growth under the low serum circumstance. Tsai CH 蔡錦華 1999 學位論文 ; thesis 61 zh-TW |
collection |
NDLTD |
language |
zh-TW |
format |
Others
|
sources |
NDLTD |
description |
碩士 === 國立臺灣大學 === 微生物學研究所 === 87 === In Epstein-Barr virus (EBV) latently infected cells, EBNA-1 proteins could bind to the specific DNA sequences as a homodimer form in order to promote viral DNA replication and to maintain the viral DNA as an episomal form. Both lines of evidence that EBNA-1 protein was only viral protein existed in most EBV-associated malignancies and that EBNA-1 could induce B lymphoma in transgenic mice model, indicated that EBNA-1 might play some roles in the carcinogenesis of EBV-associated tumors. However, it is still not clear what cellular factors would be involved or regulated in this EBNA-1-mediated pathway. The major goal of this thesis was to investigate whatever cellular molecules regulated or activated by EBNA-1 using EBNA-1-expression stable clone and transient transfection assay system.
In the preliminary results, it was observed that EBNA-1 could enhance the phosphorylation of p38 protein and increase the expression of Egr-1 in both stable and transient EBNA-1-expression RHEK-1 and 293 cells. Furthermore, the induction of Egr-1 protein by EBNA-1 could be suppressed by SB202190, a specific inhibitor for p38 phosphorylation. Both data suggested that EBNA-1 could stimulate the synthesis of Egr-1 protein through the action of phosphorylation of p38.
It has been reported that NF-κB is one of the downstream molecule of p38 protein. According to the analysis in our luciferase activity assay, the activity of NF-κB could be increased at a dose-dependent manner by the presence of EBNA-1 proteins in the transfection assay. However, it still needs more experiments to prove whether this induction of NF-κB by EBNA-1 is mediated through p38 or not.
We had found that the EBNA-1-expression RHEK-1 cells were grown better than the control RHEK-1 cells, in low serum culture condition. Here, the results of luciferase activity assay showed that both AP-1 and CRE activity were higher in the EBNA-1 expression cells at the low serum culture condition. We hypothesize that EBNA-1 could promote the cell growth under the low serum circumstance.
|
author2 |
Tsai CH |
author_facet |
Tsai CH Liu Yu-sheng 劉育昇 |
author |
Liu Yu-sheng 劉育昇 |
spellingShingle |
Liu Yu-sheng 劉育昇 Study of Signal Transduction of Epstein-Barr Virus EBNA-1 Protein in Human Epithelial Cell Lines |
author_sort |
Liu Yu-sheng |
title |
Study of Signal Transduction of Epstein-Barr Virus EBNA-1 Protein in Human Epithelial Cell Lines |
title_short |
Study of Signal Transduction of Epstein-Barr Virus EBNA-1 Protein in Human Epithelial Cell Lines |
title_full |
Study of Signal Transduction of Epstein-Barr Virus EBNA-1 Protein in Human Epithelial Cell Lines |
title_fullStr |
Study of Signal Transduction of Epstein-Barr Virus EBNA-1 Protein in Human Epithelial Cell Lines |
title_full_unstemmed |
Study of Signal Transduction of Epstein-Barr Virus EBNA-1 Protein in Human Epithelial Cell Lines |
title_sort |
study of signal transduction of epstein-barr virus ebna-1 protein in human epithelial cell lines |
publishDate |
1999 |
url |
http://ndltd.ncl.edu.tw/handle/06560713075323897358 |
work_keys_str_mv |
AT liuyusheng studyofsignaltransductionofepsteinbarrvirusebna1proteininhumanepithelialcelllines AT liúyùshēng studyofsignaltransductionofepsteinbarrvirusebna1proteininhumanepithelialcelllines AT liuyusheng ebbìngdúebna1dànbáizhìzàirénlèishàngpíxìbāozhūxùnhàochuándìzhīyánjiū AT liúyùshēng ebbìngdúebna1dànbáizhìzàirénlèishàngpíxìbāozhūxùnhàochuándìzhīyánjiū |
_version_ |
1718174736901472256 |