Study of Improving Nucleic Acid Hybridization Efficiency Utilizing Microfluidic Technology

• Main Authors: Chang-Lin Yang, 楊長霖
• Other Authors: Yu-Cheng Lin
• Format: Others
• Language: zh-TW
• Published: 2003
• Online Access: http://ndltd.ncl.edu.tw/handle/87290308073823201980

碩士 === 國立成功大學 === 工程科學系碩博士班 === 91 === The conventional hybridization method often takes 16 to 18 hours to replenish target, which is hybridized with the probe by diffusion, and impediment for large number and fast examining. The present study brings up a method to improve hybridization efficiency by a bi-directional device, which drives samples in the reaction area to flow back and forth, to replenish target, which is hybridized with probe by convection. The designed reaction area, at which the position of probe having higher extensional strain rate by back and forth target mixture flow. DNA can be stretched at this higher extensional strain rate area, and the base pair of DNA can be exposed to complementary target. Microfluidic hybridization chip is consist of a bi-directional driving device, a reaction area, spotted slide and temperature controlled device. The system can control solenoid valves, which is connected to suction and exclusion component, to fast repeat on and off to drive the sample solution in the reaction area flowing back and forth by LabVIEW controlled software. The maxmium oscillation frequency of the system is 2.25 Hz. Hybridization by conventional and microfluidic method was presented and compared in this work. Results reveal that the designed microflluidic hybridization chip can improve hybridization efficiency.