| Summary: | 碩士 === 國立臺灣大學 === 解剖學暨細胞生物學研究所 === 91 === The mutilated foot rat (mf rat) is a mutant with autosomal recessive sensory neuropathy with mutilation of the hind limbs. Decreased numbers of dorsal root ganglion cells and diminished sensitivity to painful stimuli are characteristics of these animals. We performed immnocytochemistry, to evaluate sensory and autonomic nerve of the skin in foot pad and belly. These markers included protein gene product 9.5 (PGP9.5), growth-associated protein-43 (GAP-43), substance P (SP), calcitonin gene-related peptide (CGRP), p75 nerve growth factor receptor (p75), and tyrosine hydroxylase (TH). We also performed the semithin section In comparison with wild type rats, the pad skin of mf rats had substantially fewer PGP9.5, GAP43, SP, CGRP, and TH-immunoreactive fibers. Nerve fibers immnoreactive for SP, CGRP, and TH were reduced in the belly. But the degree of fiber loss was less extensive than that in the pad. The results suggest that there is a marked reduced cutaneous innervation in foot pad skin, while it is moderate in belly skin. The number of dermal nerve bundles and the number of nerve fibers per bundle were reduced in mf rats compared to those of wild type rats. The results suggest sensory and autonomic denervation in mf rats.
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