| Summary: | 博士 === 國立臺灣大學 === 生物產業機電工程學研究所 === 92 === Abstract
Increasing gas exchange rate (E) of the TC vessel can reduce the vitrification problem frequently found in carnation (Dianthus caryophllus L. cv. Opale) shoot tip culture and adventitious buds production of Scrophularia yoshimurae. More over, higher quality of carnation shoot tip can be produced by increasing E value was found. Problem of decreasing buds counts when increasing E value can be solved by the control on the timing to initiate the gas exchange process. When 4 layers of dispense paper covered with 2 layers of parafilm was used, the highest buds count and best quality buds with no vitrification occurred can be derived if the timing to remove the parafilm to initiate the gas exchange process is set at the 4th week after transplanting.
Besides the design and installation of the transplanting room and the production line for the preparation of cultural media, filling, and sterilization of TC vessels, a 2 floors production building has been established. Within the building, movable multi-layer benches equipped with retractable light mounting fixture were installed which can increase 13 times planting area per unit land area and cost down half of the light. Fan and pad system was used to not only remove the extra thermal energy but also increase the exchange rate of gas inside and outside vessels. A high productivity and low cost plant factory for the mass production of tissue culture (TC) plantlets has been established and is currently under commercial operations.
High intensity light-emitting diode (LED) is the promising light source for TC operations. Providing with alternate current (AC), red LEDs alone can be used to grow TC plantlets of Calla Lily (Black Magic).
KEYWORD: Plant Factory, Tissue Culture, LED
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