The effect of Gelatin as scaffold for articular cartilage tissue-engineering on the growth of Chondrocytes

• Main Authors: Wei-te Li, 李偉德
• Other Authors: Ta-Jen Huang
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/32506814849177806504

碩士 === 國立清華大學 === 化學工程學系 === 93 === Abstract This research use the cheap gelatin of the native polymer as materials. We change the weight percentage of the gelatin, the reaction temperature, the type of the cross-linking reactant, make the porous gelatin scaffolds by three methods. In order to discuss the advantage and disadvantage of these three kinds of methods, we analyze the property of the scaffolds. Among this three methods, “we make the gelling first, and then cross-linking” is the best methods. The structure of the scaffold is more uniform. The ranges of the pore size is 300μm to 500μm, and it is suitable for the adhesive growth of the choncytes. We use “we make the gelling first, and then cross-linking” method to prepare GA and GP scaffolds, GA scaffold use glutaraldehyde as the cross-linking reagent, GP scaffold use genipin as the cross-linking reagent. We culture the choncytes by these two scaffolds. The results show that if the numbers of the chondrocytes which adhere to GA scaffolds is less, the chondrocytes can not secrete and synthesize collagen and glycosaminoglycan (GAG).We can observe a large amount of the chondrocytes which adhere to GP scaffolds. The chondrocytes can secrete and synthesize a large amount of collagen and glycosaminoglycan(GAG). The density of chondrocytes in GA scaffold is similar to the choncytes in normal cartilage. We seed different numbers of the chondrocytes in GP scaffold, thirty days later, the seeding numbers is 5×105 of the phenotype is spindle, and the seeding numbers is 2×106 or 5×106 of the phenotype is round. The density and the phenotype of chondrocytes is similar to the choncytes in normal cartilage. The numbers of chondrocytes have the trend of the growth, the rates of the growth decrease with time, and can secrete and synthesize extracellular matrix steadily.