Frequency Scan of a Quadrupole Mass Analyzerin the Third Stability Region for Protein Analysisand Development of Absolute Charge State Assignment Program
碩士 === 國立臺灣大學 === 物理研究所 === 93 === Part A: A linear quadrupole mass spectrometer operating in the third stability region [(a, q) ≈ (3, 3)] has been developed and utilized to obtain the mass spectra of multiply charged protein ions generated by electrospray ionization. The spectra were acquired by s...
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ndltd-TW-093NTU051980502015-12-21T04:04:15Z http://ndltd.ncl.edu.tw/handle/51238027515224302663 Frequency Scan of a Quadrupole Mass Analyzerin the Third Stability Region for Protein Analysisand Development of Absolute Charge State Assignment Program 四極柱質譜儀第三穩定區的頻率掃描與絕對電荷分析程式 Chung-Wei Lin 林崇偉 碩士 國立臺灣大學 物理研究所 93 Part A: A linear quadrupole mass spectrometer operating in the third stability region [(a, q) ≈ (3, 3)] has been developed and utilized to obtain the mass spectra of multiply charged protein ions generated by electrospray ionization. The spectra were acquired by scanning the frequency of the quadrupole with a home-built wide-band power amplifier from 400 to 100 kHz at constant voltage amplitudes in the range of 100 V. A resolution approaching R1/2 = 200 was achieved for doubly charged gramicidin S ions at m/z 570.5. Besides the experiments, a computer simulation was utilized to discuss the results of experiments. Further improvement of the mass resolution is expected when a higher voltage power amplifier is used. Part B: A new technique for determining the masses of nanoparticles in a quadrupole ion trap has been developed in our group. When a particle is introduced into the ion trap and trapped, mass-to-charge ratio of the particle is determined by varying the voltage and RF frequency. Computer programs were utilized to speed the data analysis. Two kinds of programs were compared to testify the validity of data analysis. 李遠哲 2005 學位論文 ; thesis 39 en_US |
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碩士 === 國立臺灣大學 === 物理研究所 === 93 === Part A: A linear quadrupole mass spectrometer operating in the third stability region [(a, q) ≈ (3, 3)] has been developed and utilized to obtain the mass spectra of multiply charged protein ions generated by electrospray ionization. The spectra were acquired by scanning the frequency of the quadrupole with a home-built wide-band power amplifier from 400 to 100 kHz at constant voltage amplitudes in the range of 100 V. A resolution approaching R1/2 = 200 was achieved for doubly charged gramicidin S ions at m/z 570.5. Besides the experiments, a computer simulation was utilized to discuss the results of experiments. Further improvement of the mass resolution is expected when a higher voltage power amplifier is used.
Part B: A new technique for determining the masses of nanoparticles in a quadrupole ion trap has been developed in our group. When a particle is introduced into the ion trap and trapped, mass-to-charge ratio of the particle is determined by varying the voltage and RF frequency. Computer programs were utilized to speed the data analysis. Two kinds of programs were compared to testify the validity of data analysis.
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author2 |
李遠哲 |
author_facet |
李遠哲 Chung-Wei Lin 林崇偉 |
author |
Chung-Wei Lin 林崇偉 |
spellingShingle |
Chung-Wei Lin 林崇偉 Frequency Scan of a Quadrupole Mass Analyzerin the Third Stability Region for Protein Analysisand Development of Absolute Charge State Assignment Program |
author_sort |
Chung-Wei Lin |
title |
Frequency Scan of a Quadrupole Mass Analyzerin the Third Stability Region for Protein Analysisand Development of Absolute Charge State Assignment Program |
title_short |
Frequency Scan of a Quadrupole Mass Analyzerin the Third Stability Region for Protein Analysisand Development of Absolute Charge State Assignment Program |
title_full |
Frequency Scan of a Quadrupole Mass Analyzerin the Third Stability Region for Protein Analysisand Development of Absolute Charge State Assignment Program |
title_fullStr |
Frequency Scan of a Quadrupole Mass Analyzerin the Third Stability Region for Protein Analysisand Development of Absolute Charge State Assignment Program |
title_full_unstemmed |
Frequency Scan of a Quadrupole Mass Analyzerin the Third Stability Region for Protein Analysisand Development of Absolute Charge State Assignment Program |
title_sort |
frequency scan of a quadrupole mass analyzerin the third stability region for protein analysisand development of absolute charge state assignment program |
publishDate |
2005 |
url |
http://ndltd.ncl.edu.tw/handle/51238027515224302663 |
work_keys_str_mv |
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