| Summary: | 碩士 === 實踐大學 === 食品營養研究所 === 93 === Abstract
Free radicals could caused oxidative damages in lipids, proteins, and nucleic acids and have been linked with developments of various diseases, including atherosclerosis, cancer, diabetes, aging, cataract, inflammation, and rheumatoid arthritis. Wax gourd (Benincasa hispida) has been used in traditional Chinese medicine to treat inflammation and high blood pressure. Previously studies also showed that wax gourd have antioxidation ability and angiotension converting enzyme (ACE) inhibition capacities. Lactobacillus casei is well know and useful probiotic in manufacture, and many studies has demonstrated its function in alleviate allergic and anti-inflammatory disease.
The aim of this study was to evaluate the products of wax gourd juice lactic acid bacteria fermentations relative to antioxidants contents, storage condition, and survival rate in simulation gastric acid condition.
The result showed that fermented product had higher inhibition rate of linoleic acid, oxidation and DPPH radical scavenging ability, and total phenol content than fresh wax gourd juice. The ACE activity inhibition assay was not showed significant different between the fresh wax gourd juice and fermented product. The growth rate of L. casei in fresh wax gourd juice is lower than MRS broth. The numbers of lactic acid bacteria L. casei showed no significant change in fresh wax gourd juice and MRS broth during storage for 10 days at 4℃, but L. casei cell decreased slowly in the fermented wax gourd juice held at 25℃. In simulated gastric juice assay, L. casei had better surviving rate in fresh wax gourd juice at pH 3.0 and 2.5 level for 1 h than in MRS broth in simulated gastric juice assay.
In conclusion, wax gourd juice could increase survival rate of the lactic acid bacteria L. casei during the storage and exposure to simulated gastric acid. Wax gourd juice fermented with Lactobacillus casei could increase ability of antioxidation and ACE activity inhibition which may also provide better protective effects against cardiovascular disease, and cancer.
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