The synthesis of aminated and hyaluronan coated CdSe/ZnS quantum dots and their applications

• Main Authors: Yen-Feng Lee, 李彥鋒
• Other Authors: Yng-Jiin Wang
• Format: Others
• Language: zh-TW
• Published: 2005
• Online Access: http://ndltd.ncl.edu.tw/handle/77316648976870987924

碩士 === 國立陽明大學 === 醫學工程研究所 === 93 === The aims of this study were to improve the coating process of CdSe/ZnS quantum dots, especially the covalent coupling of cysteine and hyaluronc acid. The applications of these quantum dots for cell labeling were also demonstrated. CdSe/ZnS quantum dots with sizes of about 2-5 nm were coated with cysteine and the resulted products were soluble and dispersive in aqueous buffer. The cysteine-quantum dots were delivered into U-87MG (ATCC HTB-14TM is a hypodipoid human cell line) cells by liposome transfection and the internalized quantum dots were still visible 8 days after transfection. By using EDC/NHS as coupling agent, hyaluronic acid was successfully coupled to the quantum dots. The hyaluronate -coupled quantum dots were transported into the breast cancer cells (ATCC HTB-129) which expressed large amounts of CD44. The internalized hyaluronate-quantum dots appeared to aggregate inside endosomes. The hyaluronate-quantum dots did not bind to the fibroblast cells which did not express CD44 - the receptor of hyaluronic acid. The results of this study demonstrated that hyaluronate-quantum dots have great potential for future applications of cell labeling.