THE RHIZOME TISSUE CULTURE AND ANALYSIS OF ANTIOXIDATION PROPERTY AND TECTORIGENIN OF NEOMARICA GRACILIS

• Main Authors: Hsing-Chun Hsieh, 謝幸君
• Other Authors: Chin-Wen Ho
• Format: Others
• Language: en_US
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/60061600295671749295

碩士 === 大同大學 === 生物工程學系(所) === 94 === Rhizomes were induced from in vitro seedlings of Neomarica gracilis cultured in MS basal medium contained with 30 g/l sucrose, 0.5 mg/l BA, 0.84 mg/l NAA and 0.1 mg/l 2,4-D. Fast growing tissue obtained from the rhizome tissues were subcultured in the same liquid medium and the multiplication of rhizome tissues were 4-fold after 30 days culture. The influence of jasmonic acid, l-phenylalanine and yeast extract addition on the radical scavenging activity, total flavonoid amount and tectorigenin accumulation in rhizome tissues were investigated. The radical scavenging activity under system concentration 5 mg DW/ml can increase to 90 % in methanol extract from rhizome tissues subcultured in MS basal medium contained with 30 g/l sucrose, 0.5 mg/l BA, 0.84 mg/l NAA, 0.1 mg/l 2,4-D and yeast extract (100 ~ 400 mg/l). The total flavonoid amount and tectorigenin accumulation in cell increased in methanol extract from rhizome tissues subcultured in MS basal medium contained l-phenylalanine. The maximum total flavonoid reached 1.059 % DW, when rhizome tissues subcultured in MS basal medium contained with 30 g/l sucrose, 0.5 mg/l BA, 0.84 mg/l NAA, 0.1 mg/l 2,4-D and 300 mg/l l-phenylalanine. The rhizome tissues subcultured in MS basal medium contained with 30 g/l sucrose, 0.5 mg/l BA, 0.84 mg/l NAA, 0.1 mg/l 2,4-D and 200 mg/l l-phenylalanine has the highest tectorigenin content (0.00427 % DW). The rhizome of Neomarica gracilis planted in field is unable to measure the tectorigenin.