| Summary: | 碩士 === 國立臺灣海洋大學 === 食品科學系 === 95 === The objectives of this thesis are to elucidate the physical properties and the enhancing effect of fibroblast growth in the scaffolds of yam mucoploysaccharides cross-linked with genipin or glutaraldehyde. Yam mucoploysaccharides were extracted from Dioscorea alata L. The proliferation effect of human skin fibroblasts on yam mucopolysaccharides were determined by MTT assay. The porous yam mucoploysaccharides scaffolds were prepared by freeze-drying method. Different volume ratios of cross-linking agents and yam mucopolysaccharides solution were blended and freeze-dried. The physical properties included crosslinking degree, network structure, porosity, pore sizes, swelling ratio, weight loss ratio and mechanical properties. The cell toxicity and enhancing proliferation of fibroblasts in scaffold were measured by MTT assay.
The results showed that yam mucopolysaccharides were a kind of glycoprotein. Crosslinking degree of the genipin cross-linked scaffolds increased with increasing crosslinking time, but that of glutaraldehyde cross-linked scaffolds were time independent. Crosslinking degree of scaffolds increased with increasing concentration of cross-linking reagents. SEM graph showed that the scaffolds were porous by either cross-linked with genipin or glutaraldehyde. The pore size and porosity of scaffolds varied with freezing temperatures. The pore sizes were smaller for those freezing dried at lower temperatures. The cross-linking reagents didn’t affect pore size and porosity. The swelling ratio increased at initial stage and reached to the plateau over time. The swelling ratio of the genipin cross-linked scaffolds was higher than that of the glutaraldehyde cross-linked ones. The swelling ratio of scaffolds decreased with increasing concentration of cross-linking reagents. The scaffolds degraded by lysozome over time, and the genipin cross-linked scaffolds were degraded faster than the glutaraldehyde cross-linked ones. The weight loss ratio of scaffolds decreased with increasing concentration of cross-linking reagents. The tensile strength of genipin cross-linked scaffolds was smaller than the glutaraldehyde cross-linked scaffolds, however the elongation was higher. Yam mucoploysaccharides scaffolds were no toxic for human skin fibroblasts and could enhance fibroblasts proliferation.
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