Selection of biofilm formation related-genes from a Klebsiella pneumoniae mutant library

• Main Authors: Hui-Ching Yang, 楊惠晴
• Other Authors: 王錦堂
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/36052827283316395885

碩士 === 國立臺灣大學 === 微生物學研究所 === 95 === Klebsiella pneumoniae is an opportunistic pathogen associated with nosocomial infections such as urinary tract infection, bacteremia and pneumonia. The biofilm formation of bacteria has been known to involve in the increasing resistance to antibiotic, antibacterial, and host immune responses. In order to explore the genes responsible for the biofilm formation in K. pneumoniae, a NTUH-K2044 mutant transposon library was screened by biofilm microtiter plate assay. Three mutants revealed decreased biofilm formation, and three mutants revealed increased biofilm formation compared with wild-type. The interrupted gene of one mutant with increased biofilm formation was similar with sugE in Escherichia coli. Unmarked deletion and chromosomal complementation of sugE demonstrated that sugE was responsible for the biofilm formation. In order to sutudy the role of gene regulation in sugE in biofilm formation, the RNA expression profiles of ΔsugE mutant was compared with those of wild type by microarray. The expressions of twelve clones were up-regulated in ΔsugE mutant. These twelve clones contained genes involved in maltose catabolism and carbohydrate phosphotransferase system (PTS) that had been proven to associate with biofilm formation. These results suggested that sugE might affect the biofilm formation through the regulation of genes in maltose regulon and carbohydrate phosphotransferase system.