| Summary: | 碩士 === 臺北醫學大學 === 生物醫學材料研究所 === 95 === The purpose of the study is to make the scaffolds fitting for chondrocyte to grow and let them secrete Extracellular matrix regularly to keep the development, which could attain the goal of regenerating and repaired the damaged cartilage. We construct chitosan scaffolds and analyze their Mechanical properties by electrospinning and freeze-drying, then installing chondrocyte into scaffolds to cultivate, at last, observing their development. We observe the growth state of them in different time by MTT assay, and observe their distribution in the scaffolds with H & E stain and then we analyze their characteristic of protein expresses by RT-PCR way. We use chitosan with deferent molecular weight for the material in electrospinning; and take RHIZOCHITOSAN and SACCHACHITOSAN extracting from fungus comparing to chitosan in freeze-drying.
The result shows that it is much tougher using acetic acid solution for solvent to originate Hydrophilic chitosan membrane in the experiment of electrospinning. For the boiling point of water is higher, the thermal energy generated from electrospinning process couldn’t evaporate the moisture content in the solvent completely to bring about fiber whatever even though we add other solvent or surfactant such as alcohol、PEG、DMF and NMP.
In the other aspect, sponge made by chitosan and the RHIZOCHITOSAN and SACCHACHITOSAN extracting from fungus in freeze-drying show the structure which are composed of the connected holes under SEM observe. It means that the environment is suit for developing chondrocyte; and we also found that the pore size of pure RHIZOCHITOSAN are smaller in scaffold pore size to measure. The pore size became bigger after collagen added and they became tiny when more RHIZOCHITOSAN joined. And the size of them changed with the molecular weight of chitosan. We found that adding EDC crosslinking conditions, Strain is lower obvious; in scaffold Mechanical properties and strain moved contradictorily with the molecular weight and irrelatively to RHIZOCHITOSAN. In the Force part, especially for chitosan, groups EDC added shows the fracture point occurs with the increasing strength; and in Static Stress, the RHIZOCHITOSAN is smaller（2.5~4.7*105 (Pa) ）and the chitosan is bigger (7.7~11.6*105 (Pa). Now we’ve installed the chitosan into scaffold, and the experiment is just ongoing. We look forward to the chitosan progress be prosperous.
|
|---|
