Study of the transcriptional regulation of unpaired eexpression in Drosophila eye development

碩士 === 國立陽明大學 === 生命科學暨基因體科學研究所 === 95 === unpaired (upd) encodes a secreted ligand for the Jak/STAT pathway. It is expressed at the central point of the posterior margin in the eye disc, the initiation site of the morphogenetic furrow (MF). Thus upd may play the role in MF determination. It had bee...

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Main Authors: Chuan-Ju Wang, 王釧如
Other Authors: Y. Henry Sun
Format: Others
Language:zh-TW
Published: 2007
Online Access:http://ndltd.ncl.edu.tw/handle/95291274645200234800
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spelling ndltd-TW-095YM0051050112015-10-13T14:13:12Z http://ndltd.ncl.edu.tw/handle/95291274645200234800 Study of the transcriptional regulation of unpaired eexpression in Drosophila eye development 探討upd基因在果蠅複眼發育過程中所受之轉錄調控 Chuan-Ju Wang 王釧如 碩士 國立陽明大學 生命科學暨基因體科學研究所 95 unpaired (upd) encodes a secreted ligand for the Jak/STAT pathway. It is expressed at the central point of the posterior margin in the eye disc, the initiation site of the morphogenetic furrow (MF). Thus upd may play the role in MF determination. It had been shown that several signaling pathways are involved in MF initiation, such as N, Dpp, Hh and Wg. But it is still unclear what factors specify the expression of upd. To understand the regulatory network controlling upd expression, we checked the interactions between upd and several signaling pathways. Testing of gain-of-function and loss-of-function effects of these pathways on the expression of upd-lacZ suggested Hh and Dpp pathway cooperate with N signaling to activate upd expression at the posterior center of the eye disc. Furthermore, we found that Wg signaling may negatively regulate upd expression, and Upd was also involved in auto-regulation of its own expression. We are also interesting in finding the direct regulation of upd. We checked the direct regulation by enhancer identifications and binding site mutations of several transcriptional factors. We found N, Hh and Wg signaling may regulate upd through these enhancers. In this study, we demonstrated the spatial and temporal control of upd expression. The intersection of N and Dpp, Hh signaling determines the upd expression. We also discovered a novel finding that Wg signaling restricts the upd expression in posterior center and Jak/STAT pathway may have auto-regulation to maintain upd expression. Y. Henry Sun 孫以瀚 2007 學位論文 ; thesis 0 zh-TW
collection NDLTD
language zh-TW
format Others
sources NDLTD
description 碩士 === 國立陽明大學 === 生命科學暨基因體科學研究所 === 95 === unpaired (upd) encodes a secreted ligand for the Jak/STAT pathway. It is expressed at the central point of the posterior margin in the eye disc, the initiation site of the morphogenetic furrow (MF). Thus upd may play the role in MF determination. It had been shown that several signaling pathways are involved in MF initiation, such as N, Dpp, Hh and Wg. But it is still unclear what factors specify the expression of upd. To understand the regulatory network controlling upd expression, we checked the interactions between upd and several signaling pathways. Testing of gain-of-function and loss-of-function effects of these pathways on the expression of upd-lacZ suggested Hh and Dpp pathway cooperate with N signaling to activate upd expression at the posterior center of the eye disc. Furthermore, we found that Wg signaling may negatively regulate upd expression, and Upd was also involved in auto-regulation of its own expression. We are also interesting in finding the direct regulation of upd. We checked the direct regulation by enhancer identifications and binding site mutations of several transcriptional factors. We found N, Hh and Wg signaling may regulate upd through these enhancers. In this study, we demonstrated the spatial and temporal control of upd expression. The intersection of N and Dpp, Hh signaling determines the upd expression. We also discovered a novel finding that Wg signaling restricts the upd expression in posterior center and Jak/STAT pathway may have auto-regulation to maintain upd expression.
author2 Y. Henry Sun
author_facet Y. Henry Sun
Chuan-Ju Wang
王釧如
author Chuan-Ju Wang
王釧如
spellingShingle Chuan-Ju Wang
王釧如
Study of the transcriptional regulation of unpaired eexpression in Drosophila eye development
author_sort Chuan-Ju Wang
title Study of the transcriptional regulation of unpaired eexpression in Drosophila eye development
title_short Study of the transcriptional regulation of unpaired eexpression in Drosophila eye development
title_full Study of the transcriptional regulation of unpaired eexpression in Drosophila eye development
title_fullStr Study of the transcriptional regulation of unpaired eexpression in Drosophila eye development
title_full_unstemmed Study of the transcriptional regulation of unpaired eexpression in Drosophila eye development
title_sort study of the transcriptional regulation of unpaired eexpression in drosophila eye development
publishDate 2007
url http://ndltd.ncl.edu.tw/handle/95291274645200234800
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