| Summary: | 碩士 === 國立陽明大學 === 醫學工程研究所 === 95 === In this thesis, we use au-ag core-shell bimetallic nanoparticles to achieve surface enhanced raman scattering (SERS). By using the SERS technique which provides high sensitivity for qualitative and quantitative analysis of creatinine concentration. Stable silver coated gold nanoparticle, were prepared and tested as a substrate for surface enhanced Raman scattering (SERS). Nanoparticles containing a 12 nm gold core and coated with a silver shell varying from 0–110 nm were prepared. We observed the fluctuation of SERS sensitivity depends on the mixed time and pH value.
In biomolecular detection, we applied SERS technique to analyze components of urine. By controlling the surface property of bimetal for improve linear dynamic range. We have obtained the square of correlation coefficient between SERS intensity and physiological creatinine concentration (0.5-3 mg/ml) is 0.9.
In the quantitative experiment, we found that SERS intensity is not always linearly proportional to the concentration and it was also influenced by time after mixing of colloid and sample, and the other components in the mixture. This research demonstrated the feasibility of SERS technique for measurement of urine and indicated the difficulties of quantitative analysis by SERS using bimetallic nanoparticles.
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