| Summary: | 碩士 === 國立臺灣海洋大學 === 生物科技研究所 === 96 === During embryogenesis, the processes of cell differentiation, organogenesis and response to environmental stimulation are all controlled by serious gene regulation system. The bHLH-PAS (Per-AhR/Arnt-Sim) protein family plays important roles in vertebrate development and xenobiotic metabolism. In the bHLH-PAS mediated pathway, Arnt protein plays as a central role, which can dimerize with other bHLH-PAS (such as AHR, HIF, and SIM) to regulate a variety genes.
Previous studies have shown that repression of arnt2 gene caused severe defect in neuron and axon development. Glial cell is an important role in neurogenesis. Hence, we proposed that arnt2 gene may involve in glial cell development.
In order to understand the detail regulatory mechanism, Here I have investigated the expression patterns of several glial cell markers, including olig2, sox10, mbp, nkx2.2, gfap and cyp19b, by in situ hybridization. It appeared that the oligodendrocyte marker, sox10 and olig2 have been affected significantly in spinal cord by arnt2 knockdown. Furthermore, arnt2 morpholino also influence the processes of schwann cell differentiation. Finally, blockage of arnt2 translation caused serious defect in the development of mbp in central and peripheral nervous system. In the aspect of astrocyte, arnt2 knockdown delayed astrocyte development, but did not block oligodendrocyte and astorcyte interaction obviously. Nevertheless, arnt2 knockdown did not affect the radial glia cell differentiation.
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