The function of zebrafish ARNT2 in glail cell development
碩士 === 國立臺灣海洋大學 === 生物科技研究所 === 96 === During embryogenesis, the processes of cell differentiation, organogenesis and response to environmental stimulation are all controlled by serious gene regulation system. The bHLH-PAS (Per-AhR/Arnt-Sim) protein family plays important roles in vertebrate develop...
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ndltd-TW-096NTOU51110312016-04-27T04:11:48Z http://ndltd.ncl.edu.tw/handle/21718461025213461895 The function of zebrafish ARNT2 in glail cell development 斑馬魚ARNT2基因在神經膠細胞發育的功能 Pei-Ran Liu 劉沛然 碩士 國立臺灣海洋大學 生物科技研究所 96 During embryogenesis, the processes of cell differentiation, organogenesis and response to environmental stimulation are all controlled by serious gene regulation system. The bHLH-PAS (Per-AhR/Arnt-Sim) protein family plays important roles in vertebrate development and xenobiotic metabolism. In the bHLH-PAS mediated pathway, Arnt protein plays as a central role, which can dimerize with other bHLH-PAS (such as AHR, HIF, and SIM) to regulate a variety genes. Previous studies have shown that repression of arnt2 gene caused severe defect in neuron and axon development. Glial cell is an important role in neurogenesis. Hence, we proposed that arnt2 gene may involve in glial cell development. In order to understand the detail regulatory mechanism, Here I have investigated the expression patterns of several glial cell markers, including olig2, sox10, mbp, nkx2.2, gfap and cyp19b, by in situ hybridization. It appeared that the oligodendrocyte marker, sox10 and olig2 have been affected significantly in spinal cord by arnt2 knockdown. Furthermore, arnt2 morpholino also influence the processes of schwann cell differentiation. Finally, blockage of arnt2 translation caused serious defect in the development of mbp in central and peripheral nervous system. In the aspect of astrocyte, arnt2 knockdown delayed astrocyte development, but did not block oligodendrocyte and astorcyte interaction obviously. Nevertheless, arnt2 knockdown did not affect the radial glia cell differentiation. Chin-Hwa Hu Wen–Shiung Tsou 胡清華 鄒文雄 2008 學位論文 ; thesis 72 zh-TW |
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碩士 === 國立臺灣海洋大學 === 生物科技研究所 === 96 === During embryogenesis, the processes of cell differentiation, organogenesis and response to environmental stimulation are all controlled by serious gene regulation system. The bHLH-PAS (Per-AhR/Arnt-Sim) protein family plays important roles in vertebrate development and xenobiotic metabolism. In the bHLH-PAS mediated pathway, Arnt protein plays as a central role, which can dimerize with other bHLH-PAS (such as AHR, HIF, and SIM) to regulate a variety genes.
Previous studies have shown that repression of arnt2 gene caused severe defect in neuron and axon development. Glial cell is an important role in neurogenesis. Hence, we proposed that arnt2 gene may involve in glial cell development.
In order to understand the detail regulatory mechanism, Here I have investigated the expression patterns of several glial cell markers, including olig2, sox10, mbp, nkx2.2, gfap and cyp19b, by in situ hybridization. It appeared that the oligodendrocyte marker, sox10 and olig2 have been affected significantly in spinal cord by arnt2 knockdown. Furthermore, arnt2 morpholino also influence the processes of schwann cell differentiation. Finally, blockage of arnt2 translation caused serious defect in the development of mbp in central and peripheral nervous system. In the aspect of astrocyte, arnt2 knockdown delayed astrocyte development, but did not block oligodendrocyte and astorcyte interaction obviously. Nevertheless, arnt2 knockdown did not affect the radial glia cell differentiation.
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author2 |
Chin-Hwa Hu |
author_facet |
Chin-Hwa Hu Pei-Ran Liu 劉沛然 |
author |
Pei-Ran Liu 劉沛然 |
spellingShingle |
Pei-Ran Liu 劉沛然 The function of zebrafish ARNT2 in glail cell development |
author_sort |
Pei-Ran Liu |
title |
The function of zebrafish ARNT2 in glail cell development |
title_short |
The function of zebrafish ARNT2 in glail cell development |
title_full |
The function of zebrafish ARNT2 in glail cell development |
title_fullStr |
The function of zebrafish ARNT2 in glail cell development |
title_full_unstemmed |
The function of zebrafish ARNT2 in glail cell development |
title_sort |
function of zebrafish arnt2 in glail cell development |
publishDate |
2008 |
url |
http://ndltd.ncl.edu.tw/handle/21718461025213461895 |
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