| Summary: | 碩士 === 中華醫事科技大學 === 生物科技研究所 === 97 === This research use Taiwan Japonica Glutinous 3 (TJG3), Tainan Japonica 11 (TJ11) and Tainan Japonica Breed (TJB) brown rice, as the material to prepare germinated brown rice, will study the effect of three pH value water（tap water, pH 5.5, pH 9.0）, three water temperature（28, 32, 36 ℃）and different soak time 4, 6, 8, 24 hr, on γ-aminobutyric acid (GABA) formation. The result in the GABA content of TJB pH 9.0 / 36 ℃ / 6 hr is the highest (17.79 mg/100 g), the following is pH 5.5 / 28 ℃ / 6 hr (16.53 mg/100 g). Add L-Glutamic acid and Chitosan really sees a significantly increasing from 9.57 mg/100 g to 16 mg/100 g. PH 5.5 groups don’t show significantly difference. While increased the Toona sinensis Roemor dosage to above 1%, reduce GABA content from 3.1 mg/100 g to 5.69 mg/100 g, verify might Toona sinensis Roemor may inhibit brown rice germinated and then reduce the formation of GABA.
Analysis of Microorganisms, Total aerobic plate counts and Total anaerobic plate counts of all group except GCTS(1 %)(include L-Glutamic acid, Chitosan and 1 % Toona sinensis Roemor) increased from 106 cfu/g to 109 cfu/g over 21 days. Total aerobic plate counts and Total anaerobic plate counts of GCTS(1 %) increased from 107 cfu/g to 109 cfu/g. The MPN of E. coli was below 3 within seven days. Coliform of GCTS(1 %, 5 %) increases period from 107 MPN/g to 102 MPN/g. At the same time, cause lower pH value and higher titratable acidity.
On the aspect of antioxidative activity, it show strong antioxidative activity on DPPH scavenging activity, ‧OH radical scavenging activity, TEAC assay when Toona sinensis Roemor added over 1 %, this expressed the antioxidative activity come from Toona sinensis Roemor. Especially that GCTS(5 %) elevate DPPH scavenging activity over 40 %. The group with TS also reduce IC50 of ‧OH radical scavenging activity, therefore, added 1 % and 5 % Toona sinensis Roemor group is might have more antioxidant compositions. On the aspect of TEAC assay, 1 and 5 %TS groups also were over 0.6 mM trolox equivalent, it was almost 2 fold compared with GC(include L-Glutamic acid, Chitosan) group.
Finally, the germinated brown rice has better acceptance degree, the same with white rice, on sensory evaluation. Brown rice has bad acceptance degree(P＜0.05).
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