Purification and Characterization of a New Rhizopuspepsin from Rhizopus oryzae NBRC 4749 and Expression of Staphylococcus aureus BCRC 15205 Glutamyl Endopeptidase Gene in Bacillus subtilis
博士 === 國立中興大學 === 分子生物學研究所 === 97 === A secretory aspartic protease (also termed as rhizopuspepsin) was purified from Rhizopus oryzae NBRC 4749 by ion exchange chromatography with a yield of 45%. The enzyme was a non-glycoprotein with a molecular mass of 37 kDa as determined by SDS-PAGE analysis. N-...
Main Authors: | , |
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Other Authors: | |
Format: | Others |
Language: | zh-TW |
Published: |
2009
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Online Access: | http://ndltd.ncl.edu.tw/handle/23816018774557892632 |