The Mechanisms of Honokiol Inhibits Tumour Growth and Angiogenesis in Gastric Cancer Cells
碩士 === 國立中興大學 === 醫學科技研究所 === 97 === Background and Aims: Honokiol, a small molecular natural product, has been shown to possess potent anti-neoplastic and anti-angiogenic properties. However, the molecular mechanism underlying the anticancer activity is poorly understood. We examined the in vitro a...
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ndltd-TW-097NCHU51140042016-04-29T04:19:43Z http://ndltd.ncl.edu.tw/handle/19550960292844893265 The Mechanisms of Honokiol Inhibits Tumour Growth and Angiogenesis in Gastric Cancer Cells 和厚朴酚抑制胃癌腫瘤細胞生長及血管新生機制之探討 Chih-Chien Wang 王致堅 碩士 國立中興大學 醫學科技研究所 97 Background and Aims: Honokiol, a small molecular natural product, has been shown to possess potent anti-neoplastic and anti-angiogenic properties. However, the molecular mechanism underlying the anticancer activity is poorly understood. We examined the in vitro and in vivo effects of PPAR-gamma and c-Met on cell growth, angiogenesis of gastric cancer and the cancer regulated genes-C/EBP beta. Methods: Four gastric cancer cell lines were used. Immunohistochemistry and immunoblotting were used to assess PPAR-gamma and c-Met expression levels in xenograft gastric cancer tumour model and cancer cell lines, respectively. MTT assay, flow cytometry and soft agar assay were used to examine tumour growth. HUVECs tube formation, rat aorta ring assay were employed to assess anti-angiogenic effect. Gelatin zymography, transwell chamber were utilized to investigates invasion and migration. Tumour growth and metastasis were analyzed in mice injected with pure compound Honokiol administered. Moreover, identification of C/EBP beta phosphorylation and translocation were analyzed by immunoprecipitation, nuclear/cytosol protein extraction and immunofluorescence staining assay. Results: PPAR-gamma, c-Met and C/EBP beta overexpression were found in gastric cancer model in vitro and in vivo. Honokiol significantly inhibited tumour growth, migration, invasion and tube formation. In xenograft gastric cancer model,we found Honokiol completely abolished peritoneal metastasis and angiogenesis better than subcutaneous suppressed tumor burden. Activation of calpain II , dephosphorylation and translocation change of C/EBP beta by Honokoil were able to down regulate PPAR-gamma, c-Met and C/EBP beta expression. Conclusions: A novel molecular Honokiol, inhibits tumor growth and angiogenesis, may be a promising drug candidate in anti-angiogenesis and anticancer agent. Meei-Ling Sheu 許美鈴 學位論文 ; thesis 73 zh-TW |
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碩士 === 國立中興大學 === 醫學科技研究所 === 97 === Background and Aims: Honokiol, a small molecular natural product, has been shown to possess potent anti-neoplastic and anti-angiogenic properties. However, the molecular mechanism underlying the anticancer activity is poorly understood. We examined the in vitro and in vivo effects of PPAR-gamma and c-Met on cell growth, angiogenesis of gastric cancer and the cancer regulated genes-C/EBP beta. Methods: Four gastric cancer cell lines were used. Immunohistochemistry and immunoblotting were used to assess PPAR-gamma and c-Met expression levels in xenograft gastric cancer tumour model and cancer cell lines, respectively. MTT assay, flow cytometry and soft agar assay were used to examine tumour growth. HUVECs tube formation, rat aorta ring assay were employed to assess anti-angiogenic effect. Gelatin zymography, transwell chamber were utilized to investigates invasion and migration. Tumour growth and metastasis were analyzed in mice injected with pure compound Honokiol administered. Moreover, identification of C/EBP beta phosphorylation and translocation were analyzed by immunoprecipitation, nuclear/cytosol protein extraction and immunofluorescence staining assay. Results: PPAR-gamma, c-Met and C/EBP beta overexpression were found in gastric cancer model in vitro and in vivo. Honokiol significantly inhibited tumour growth, migration, invasion and tube formation. In xenograft gastric cancer model,we found Honokiol completely abolished peritoneal metastasis and angiogenesis better than subcutaneous suppressed tumor burden. Activation of calpain II , dephosphorylation and translocation change of C/EBP beta by Honokoil were able to down regulate PPAR-gamma, c-Met and C/EBP beta expression. Conclusions: A novel molecular Honokiol, inhibits tumor growth and angiogenesis, may be a promising drug candidate in anti-angiogenesis and anticancer agent.
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author2 |
Meei-Ling Sheu |
author_facet |
Meei-Ling Sheu Chih-Chien Wang 王致堅 |
author |
Chih-Chien Wang 王致堅 |
spellingShingle |
Chih-Chien Wang 王致堅 The Mechanisms of Honokiol Inhibits Tumour Growth and Angiogenesis in Gastric Cancer Cells |
author_sort |
Chih-Chien Wang |
title |
The Mechanisms of Honokiol Inhibits Tumour Growth and Angiogenesis in Gastric Cancer Cells |
title_short |
The Mechanisms of Honokiol Inhibits Tumour Growth and Angiogenesis in Gastric Cancer Cells |
title_full |
The Mechanisms of Honokiol Inhibits Tumour Growth and Angiogenesis in Gastric Cancer Cells |
title_fullStr |
The Mechanisms of Honokiol Inhibits Tumour Growth and Angiogenesis in Gastric Cancer Cells |
title_full_unstemmed |
The Mechanisms of Honokiol Inhibits Tumour Growth and Angiogenesis in Gastric Cancer Cells |
title_sort |
mechanisms of honokiol inhibits tumour growth and angiogenesis in gastric cancer cells |
url |
http://ndltd.ncl.edu.tw/handle/19550960292844893265 |
work_keys_str_mv |
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