| Summary: | 碩士 === 國立臺北科技大學 === 生物科技研究所 === 97 === The purpose of this research was to develop a crosslinked chitosan ( CS ) and aliginate ( A ) porous scaffold that can be suitable for controlled drug release. Four materials made porous scaffolds were 1. chitosan ( CS ) , 2. chitosan crosslinked with alginate ( CSA ) , 3. chitosan crosslinked with alginate and Ca2+ ( CSACa ) , and 4. alginate crosslinked with Ca2+ ( ACa ). Esperiments performed on the crosslinked materials and scaffolds that physical properties were examined and in vitro effects of anti-inflammatory. The FTIR results showed Amide bonds were formed between CS and A because of the ionic attraction between NH3+ and COO-. Also, the ionic bonding between calcium ion and the alginate carbonyl group shifted the carboxyl peak to higher wavenumber. The calcium ion also effectively bonded to the COO- and improved the CSA mixture crosslinking. SEM showed CS crosslinked with A scaffold had smaller pores and more compact structure. CS hybrid scaffolds had stronger mechanical property ( higher Young’s modulus ) and less susceptible to enzymatic degradation than CS scaffolds. The hydrophilicity of CS increased after it was crosslinked with alginate. However, after crosslinked with Ca2+ the hydrophilicity of CSA decreased. Scaffolds made of CS crosslinked with A that change scaffold mechanical and hydrophilicity had less swell ratio than CS scaffolds. The release rate of PTX of CSACa slowest compared with the others groups. When the four groups of scaffolds were loaded with PTX and cultured with lipopolysaccharide-activated macrophages, the CSACa group had the best results in suppressing the release of inflammatory factors ( TNF-α and IL-6 ) after 6 and 24 hours. The slow PTX release from the CSACa scaffolds was thought to be the reason that improved the anti-inflammatory effect of PTX. We have successfully developed a tissue engineered scaffold that could not only used in controlled release but also has more effectively suppress inflammation.
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