The fermentation products of lactic acid bacteria applied in cosmetics
碩士 === 嘉南藥理科技大學 === 化妝品科技研究所 === 98 === The objective of this study is to investigate the effects of the products of soymilk fermented with lactic acid bacteria on the cultured mouse 3T3 fibroblasts, and the assays, including cell viability, the level of collagen accumulation, and the activities o...
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ndltd-TW-098CNUP57930022015-10-13T19:06:37Z http://ndltd.ncl.edu.tw/handle/18042717743130182211 The fermentation products of lactic acid bacteria applied in cosmetics 乳酸菌發酵產物應用於化粧品之研究 You-Jie Li 李宥潔 碩士 嘉南藥理科技大學 化妝品科技研究所 98 The objective of this study is to investigate the effects of the products of soymilk fermented with lactic acid bacteria on the cultured mouse 3T3 fibroblasts, and the assays, including cell viability, the level of collagen accumulation, and the activities of matrix metalloproteinases (MMPs) secreted from 3T3 fibroblasts, were performed. The anti-oxidative effect of soymilk-fermented products (SFP) was also evaluated. Besides, the efficacy of lotion containing SFP was further assayed in vivo. The date presented here showed that 48 h-treatment of 3T3 fibroblasts in the culture medium with 0.78~3.13 mg/ml of SFP could bring about at least a 30% increase in cell proliferation. In addition, SFP (3.13 mg/ml) could not directly inhibit the activities of MMP-2 and MMP-9 in the presence of culture medium. However, it also found that 48 h-treatment of 3T3 fibroblasts with 3.13 mg/ml of SFP could increase the lever of collagen accumulation by 15.11%. Furthermore, within 8h, SFP could effectively give rise to an accumulation of the content of collagen. In antioxidation, 20 mg/ml of SFP showed the scavenging effects on DPPH free radicals over 40%. On the other hand, in the in-vivo efficacious evaluation, the lotion containing 3.44 mg/ml of SFP dramatically decreased pore counts, wrinkles, textures, UV spots, and melanin by 29.36%, 14.59%, 20.36%, 1.57%, and 9.99%, respectively, in 4 weeks. Based on the fore-mentioned data, we suggest that SFP can be used as a functional cosmetic ingredient. Chau-Chen Yang 楊朝成 2010 學位論文 ; thesis 99 zh-TW |
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碩士 === 嘉南藥理科技大學 === 化妝品科技研究所 === 98 === The objective of this study is to investigate the effects of the products of soymilk fermented with lactic acid bacteria on the cultured mouse 3T3 fibroblasts, and the assays, including cell viability, the level of collagen accumulation, and the activities of matrix metalloproteinases (MMPs) secreted from 3T3 fibroblasts, were performed. The anti-oxidative effect of soymilk-fermented products (SFP) was also evaluated. Besides, the efficacy of lotion containing SFP was further assayed in vivo.
The date presented here showed that 48 h-treatment of 3T3 fibroblasts in the culture medium with 0.78~3.13 mg/ml of SFP could bring about at least a 30% increase in cell proliferation. In addition, SFP (3.13 mg/ml) could not directly inhibit the activities of MMP-2 and MMP-9 in the presence of culture medium. However, it also found that 48 h-treatment of 3T3 fibroblasts with 3.13 mg/ml of SFP could increase the lever of collagen accumulation by 15.11%. Furthermore, within 8h, SFP could effectively give rise to an accumulation of the content of collagen. In antioxidation, 20 mg/ml of SFP showed the scavenging effects on DPPH free radicals over 40%.
On the other hand, in the in-vivo efficacious evaluation, the lotion containing 3.44 mg/ml of SFP dramatically decreased pore counts, wrinkles, textures, UV spots, and melanin by 29.36%, 14.59%, 20.36%, 1.57%, and 9.99%, respectively, in 4 weeks.
Based on the fore-mentioned data, we suggest that SFP can be used as a functional cosmetic ingredient.
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author2 |
Chau-Chen Yang |
author_facet |
Chau-Chen Yang You-Jie Li 李宥潔 |
author |
You-Jie Li 李宥潔 |
spellingShingle |
You-Jie Li 李宥潔 The fermentation products of lactic acid bacteria applied in cosmetics |
author_sort |
You-Jie Li |
title |
The fermentation products of lactic acid bacteria applied in cosmetics |
title_short |
The fermentation products of lactic acid bacteria applied in cosmetics |
title_full |
The fermentation products of lactic acid bacteria applied in cosmetics |
title_fullStr |
The fermentation products of lactic acid bacteria applied in cosmetics |
title_full_unstemmed |
The fermentation products of lactic acid bacteria applied in cosmetics |
title_sort |
fermentation products of lactic acid bacteria applied in cosmetics |
publishDate |
2010 |
url |
http://ndltd.ncl.edu.tw/handle/18042717743130182211 |
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