Elucidation of biological implications of the proteomic profile changes in the early stage of repair processes of SCI rat

• Main Authors: Yi-Wen Fang, 方怡文
• Other Authors: Kin-Fu Chak
• Format: Others
• Language: zh-TW
• Published: 2010
• Online Access: http://ndltd.ncl.edu.tw/handle/81044669850491727423

碩士 === 國立陽明大學 === 生化暨分子生物研究所 === 98 === Acidic fibroblast growth factor (aFGF), a potent neurotrophic factor, could promote the survival of neurons and re-growth of neurite in the injured spinal cord. However, comprehensive mechanism of protective effect of aFGF is still remained to be resolved. Previous studies showed that significant improvement in the locomotor behavior analysis resulted from of the aFGF-treated group after contusive spinal cord injury (SCI). SCI will initiate the different pathological changes along with time, it is therefore, we would like to propose that aFGF may play different role at different time course during the repair processes. Proteomic and bioinformatic approach were adapted to investigate the protein profile changes of the damaged spinal cord tissue of the SCI rats treated either with or without aFGF at 3 days after injury. Result of proteomic analysis indicated that all these differentially expressed proteins were correlated with function of oxidative stress, anti¬apoptosis effect, neuronal transporters, and glucose metabolism, etc. Hence, the protective effect of aFGF at the time course came from a set of teamwork of multiple factors that may attenuate secondary injury for providing a better condition recovering from the damage. Furthermore, according to the result of lipid peroxidation assay, the overproduction of ROS level was indeed reduced by aFGF treatment in SCI rat. Thus, aFGF may play a role as a modulator in regulating cell oxidative stress at 3 day after SCI. In this study, our results provide a better insight into the regeneration mechanisms in the early stage of repair processes of SCI rat. We hope that our experimental results could provide insight into the molecules basis of the repair process of SCI that may be useful for clinical application for the SCI patient.