Integration of Design, Implementation, manipulation, and Testing in Lab-on-a-chip

• Main Authors: Ting-Jou Ding, 丁挺洲
• Other Authors: Tsung-Hsun Yang
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/65935900880802523867

博士 === 國立中央大學 === 光電科學研究所 === 100 === Electrowetting on dielectric (EWOD) microfluidic system takes more advantages over than the conventional continuous fluidic systems on the discrete droplets and the virtual fluid channel construction. the design of discrete droplets and the virtual fluidic channels can operate microfluidics without the pump, the valves, and the fixed microchannels. In this thesis, it is explored with the integration of the actuators and the sensors in a Lab-on-a-chip system to fit the wide reuqests in need of small volume but high diversity of interaction smaples in many applications. Actually, the integration Lab-on-a-chip system is consisted of micro-sensors, micro-actuators, and process controllers. With consideration of the easy integration, the guided-mode resonance (GMR) sensors are choosen as being the micro-sensors, and the EWOD microfluidic drivers as being the micro-actuators. It is demonstrated that the synthesis of peptides is performed on the integration Lab-on-a-chip with high production efficiency. With aids of ESCA, the production efficiency is found around 65%~75%. Besides, it is also successfully demonstrated the microsensors can be further manufactered in the Lab-on-a-chip system itself. The periodic structure in the GMR sensors is easily and efficiently produced by combining the EWOD actuating and the self-synthesis technology. As a result, it proposes one new Lab-on-a-chip that is not only with combination amoung micro-sensors, micro-actuators, and process controllers, but also the micro-sensors needed are fabricated on site by the Lab-on-a-chip itself.