Surface Acoustic Wave and Molecularly Imprinted PolymerChip of Creatine Kinase-MM and Microchannels

• Main Authors: Yu-sheng Chen, 陳昱陞
• Other Authors: Chung-yih Wang
• Format: Others
• Language: zh-TW
• Published: 2012
• Online Access: http://ndltd.ncl.edu.tw/handle/20920357660571294203

碩士 === 大同大學 === 生物工程學系(所) === 100 === We use a MEMS process to manufacture microfluidic separation chip, which is used for the pre-treatment of sample containing creatine kinase. The microfluidic chip is made of poly - dimethylsiloxane ( PDMS ). We also develop a surface modification process using the combination of oxygen plasma to extend the hydrophilicity of the PDMS material. After injection of the micro-biological samples, the microfluidic chip drives the magnetic material, such as red blood cells, to separate channel, which may reduce the interferences resulting from of those materials. Surface acoustic wave chip was made with lithium tantalite. The interdigital transducers with a width of 5 μm and a thickness of 75 nm were made with lithography technology on lithium tantalite. Creatine kinase molecules were used as template and a molecular imprinting film was prepared on the surface acoustic wave device. The holes on the molecular imprinting film are able to recognize creatine kinase. A network analyzer instrument was used to detect the changes of center frequency of surface acoustic wave when binding and re-binding of creatine kinase. The frequency changes corresponding to the concentration of creatine kinase were discussed.