| Summary: | 碩士 === 國立陽明大學 === 生化暨分子生物研究所 === 100 === Lactic acid bacteria (LAB) isolated from many kind of traditional fermented foods in Taiwan, such as sticky tofu, suan-tsai, fu-tsai, millet alcoholic beverage and black bean sauce, were investigated for their immunomodulating properties. Human peripheral blood mononuclear cells (PBMC) were treated with 100 different stains of heat-killed LAB. The cell proliferation and cytokines production of interferon- γ (IFN-γ) were examined to evaluate the in vitro immunomodulatory activities of promoting T-helper-1 (Th1) dominated cytokine production with relatively high levels of IFN-γ. After 48 hours, The LAB 237, Lactobacillus plantarum, induced PBMC with the viability greater than 80% compared to medium as control produce relatively high levels of IFN-γ. Orally administrated with three doses of LAB 237 (105, 107, and 109 CFU) in ovalbumin (OVA)-allergic asthma mice further evaluated the immunomodulating properties of LAB 237. The result showed that the level of IgE and OVA-Ige, OVA-IgG2a in serum were decreased and the level of OVA-IgG1 in serum was increased in 237-H (109 CFU) group. Oral administration of 237-H not only attenuated the airway responesssive to methacholine and reduced the number of inflammatory cells influx to airway, but also changed the cytokine profile in bronchoalveolar fluid (BALF) and in splenocytes suspension. The production of Th2 cytokines (IL-4, IL-5, and IL-13) and pro-inflammatory cytokines (IL-6, and TNF-α) were decreased significantly, and Th1 cytokines (IL-2, and IFN-γ) were increased obviously. The present results suggested that LAB 237, Lactobacillus plantarum, isolated from traditional fermented food in Taiwan had immunomodulatory activities could stimulate the cytokines production of toward a Th1 response to suppress Th2 response hence the LAB may be applicable in improving allergy symptoms in human.
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