Inhibitory effects of miR-622 on tumor invasion and migration in lung cancer cells in vitro

• Main Authors: Chung-Chih Weng, 翁崇智
• Other Authors: 鄭鈞文
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/20280045197307327413

碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 101 === Hypoxia-inducible factor-1α (HIF-1α) has been found to involves in tumor progression, angiogenesis, metastasis, and invasion. HIF-1α have also been shown to induce EMT and metastasis through the repression of E-cadherin. MicroRNAs (miRNAs) are a class of small noncoding RNAs that control gene expression by targeting mRNA 3’untranslated region. Recently, there are growing evidence showed that miRNAs can affect the genesis and development of tumor and play a kind of tumor suppressor or oncogenic function. In the present study, we identified that Hypoxia-inducible factor-1α (HIF-1α) is a putative target of hsa-miR-622.Using luciferase reporter assay we confirmed that miR-622 could reduce luciferase expression by binding HIF-1α 3’UTR. Overexpression of miR-622 in the lung cancer cell lines, A549 or H1299, HIF-1α protein level is downregulated by miR-622. Further, study in vitro has been shown that both capabilities of tumor invasiveness and motility were significantly inhibited by overexpression of miR-622 in lung cancer cells. In addition, we found that the levels of vimentin were drastically decreased, but levels of E-cadherin were induced in miR622-transfected cells. Our data indicate that miR-622 plays a key role in controlling the expression of HIF-1α may mediate the tumor suppressor effects of miR-622.