The study of CaORF19.1105 and CaORF19.5285 null mutations on the morphogenesis and phenotype of Candida albicans

• Main Authors: Tang, Tzu-chiao, 唐子喬
• Other Authors: Yang, Yun-Liang
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/53832462268892791706

碩士 === 國立交通大學 === 生物科技學系 === 103 === Candida albicans is an opportunistic pathogen, living in human oral cavity gastrointestinal tract and vaginal. For immunocompromised host, it had chance to cause lethal system infection. Candida albicans with the ability to switch morphology had high virulence. Previous study found that EFG1 and CPH1, two transcription factor, are related to morphology ability. Previous research using Microarray method to find out the gene related to morphology and drug resistance by compare wild type SC5314, JKC19 (cph1/cph1) and HLC52 (efg1/efg1). From those result, I chose CaORF19.1105 and CaORF19.5285 as my research target. CaORF19.1105 is gene with un-know function but suspected to be involved with phenotype switching. Blast result also showed that it may be a transcription factor. CaORF19.5285 is similar to PST2 gene, a Saccharomyces cerevisiae gene which may associate to ROS resistance. I Used SAT1 flipper cassette to knock-out CaORF19.1105 and CaORF19.5285 gene. Tests show both null mutants still can switch morphology. In oxygen stress test, CaORF19.5285 null mutant shows no difference when compared to the wild type. Those result mean there two genes do not influence the morphology, and CaORF19.5285 null mutant has no effect to ROS.