| Summary: | 碩士 === 國立臺灣科技大學 === 化學工程系 === 103 === Cyanophycin (CGP) and hyaluronic acid (HA) are capable of good biocompatibility and biodegradability, and there are pros and cons of both biomaterials. The structure of CGP is positive charged, favorable for cell-adhesion, under neutral environment, but the limitations of water solubility and mechanical strength result in the applications are yet widely available. HA is flexible, hydrophilic and similar to the structure of extracellular matrix, while the shortage of cell-adhesion for polysaccharide needs to be improved in tissue engineering. Therefore, we crosslinked CGP and HA with glutaraldehyde in different ratio in this study to form HA-CGP grafted scaffolds. We looked forward to combining the advantages of them to expand the applications.
In this study, we used EDC/NHS as crosslinker to produce HA-CGP conjugates in different ratio, estimating the grafted level by TNBSA assay, and the biocapabilities was determined by MTT assay. Then, the HA-CGP scaffolds were crosslinked with glutaraldehyde, observing the surface and cross-section of scaffolds with SEM, using fluorescent method and fluorescence microscopy to observe the crosslinking results, and test the swelling ratio in different points.
According to the results, the relative viability of HA and CGP are 2.26 and 0.77 times higher than control relatively, and there was the highest relative viability of HA-CGP40%, 1.17 times higher than control, of different ratio HA-CGP conjugates. In other words, compared the other ratio HA-CGP conjugates, HA-CGP40% could supply the most suitable environment for cells' proliferation. The structures of HA-CGP grafted scaffolds on surface yet differed from those on cross section significantly, and the pore size and diameter range of HA-CGP grafted scaffolds got larger with the CGP content increased. The fluorescent intensity also went up with the higher CGP content. After being immersed in 1×PBS for 24 hours, the swelling ratio of HA-CGP grafted scaffolds increased significantly.
Based on the above results, we concluded that the range of pore size and diameter of the HA-CGP grafted scaffolds is helpful for nutrients and growth factors transition, and good water retention which made the structure of scaffolds complete in medium.
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