Characterization of stem/progenitor cells in mouse ovarian surface epithelium

• Main Authors: Zi-Yu Lin, 林子淯
• Other Authors: Chun-Ming Chen
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/72016128522304929702

碩士 === 國立陽明大學 === 生命科學系暨基因體科學研究所 === 103 === Epithelium ovarian cancer (EOC) is a deadly disease and its pathogenesis is poorly understood. In this study, we used a cell-lineage tracing approach to identify a population of ovarian surface epithelium (OSE), which expresses anti-Müllerian hormone type II receptor (AMHR2) in Amhr2-Cre/+;R26R-mTmG/+ reporter mice. We found that the Amhr2-Cre positive cells were distributed in a mosaic pattern throughout the OSE. By quantification of Amhr2-Cre+ derivatives in the OSE of Amhr2-Cre/+;R26R-mTmG/+ reporter mice, we showed that the GFP+ cells accounted for 30% of OSE at the age of 3 and 5 months. This observation indicates that AMHR2+ population in OSE is essentially stable through several rounds of natural ovulation in Amhr2-Cre/+;R26R-mTmG/+ reporter mice at the age of 3- and 5-month-old. Using BrdU pulse–chase method for 3-month trace, we found that the OSE of Amhr2-Cre positive lineage had more BrdU label-retaining cells (LRCs) in comparison with that of Amhr2-Cre negative lineage. Besides, using OSE colony forming assay, we found that Amhr2-Cre+ derived colonies could be passaged to second generation, indicating that Amhr2-Cre+ derivatives of OSE may contain a stem/progenitor population.